Mouse cardiac IL-1β, IL-6 levels were measured using R&D ELISA kits, TNF-α was measured via eBioscience ELISA kit. The levels of mice IL-18 were measured using RayBiotech ELISA kit. The levels of mice LDH were measured with a biochemical analyzer (Mindray, Shenzhen, China).
Biochemical analyzer
Manufactured by Mindray
Sourced in China
The Biochemical Analyzer is a laboratory instrument designed to quantitatively measure the concentration of specific analytes in biological samples, such as blood or urine. The core function of the device is to perform automated chemical analysis of these samples, providing accurate and reliable results to support clinical diagnosis and patient management.
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Lab products found in correlation
7 protocols using biochemical analyzer
1
Cardiac Cytokine and LDH Measurement
2
Serum Cytokine and Liver Enzyme Levels
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Serum tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-4 (IL-4), and interleukin-10 (IL-10) were measured using enzyme-linked immunosorbent assay kits (Huaying Biological Technology Co., Ltd., Beijing, China).
The levels of serum aspartate transferase (AST) and alanine transaminase (ALT) were determined with a biochemical analyzer (Mindray Biomedical Electronics Co., Ltd., Shenzhen, China) and a liver function test kit (Yapu Biological Technology Co., Ltd., Shanghai, China). The intra- and interassay coefficients of variation were 8.76 and 4.46%, respectively.
The levels of serum aspartate transferase (AST) and alanine transaminase (ALT) were determined with a biochemical analyzer (Mindray Biomedical Electronics Co., Ltd., Shenzhen, China) and a liver function test kit (Yapu Biological Technology Co., Ltd., Shanghai, China). The intra- and interassay coefficients of variation were 8.76 and 4.46%, respectively.
3
Plasma Lipid Profiling in LDLr Mice
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Plasma samples were collected from LDLr-/- mice and WT mice for lipids measurement. Total cholesterol (TC), total triglyceride (TG), low-density lipoprotein-cholesterol (LDL-C) and high-density lipoprotein-cholesterol (HDL-C) in mouse plasma were assayed with corresponding assay kit (Mindray, China) by using biochemical analyzer (Mindray, China).
4
Lipid and Hormone Profile in Mice
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At the 16th week, after being fasted for 8 h, the mice were anaesthetized with pentobarbital sodium and the blood samples were collected from eyes. The blood was centrifuged to separate the mice serum. Triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and glucose (Glu) was measured with biochemical analyzer (Mindray Bio Medical Electronics Co., Ltd.). Serum testosterone (CUSABIO Biotech Co., Ltd., Wuhan, China) and serum luteinizing hormone (Cloud-Clone Corp, Houston, USA) levels were measured by an enzyme-linked immunosorbent assay (ELISA) kit according to the manufacturer's protocols.
5
Biochemical Analysis of Metabolites
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Biochemical metabolites in plasma were determined using a biochemical analyzer (Mindray, Shenzhen, China). Glycogen contents were determined in livers and muscles (n = 6) using commercial kits (Liver/muscle glycogen kit, Jiancheng Bioengineering Institute, Nanjing, China). The activity of caspase 3 were determined in livers using commercial kits (Caspase 3 Activity Assay Kit, Beytime Biotechnology, Shanghai, China).
6
Serum Biomarker Measurement Protocol
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Serum aspartate amino transferase (AST) was measured with Biochemical Assay Kit (Mindray, Cat.105-000442-00/105-000443-00), according to the manufacturer’s instruction. Blood total cholesterol (TC) and low-density lipoprotein-cholesterol (LDL-C) levels were measured with Biochemical analyzer (Mindray BS240) according to the manufacturer’s instruction.
7
Measuring Blood Lipid Profile
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Blood lipids including total cholesterol (TC), total triglyceride (TG), low-density lipoprotein-cholesterol (LDL-C), and high-density lipoprotein-cholesterol (HDL-C) were measured with corresponding assay kit (Mindray, China) using biochemical analyzer (Mindray, China).
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