The cardiotoxicity indices including brain natriuretic peptide (BNP) and cardiac troponin I (cTnI) were determined in serum using a rat ELISA kit (Cloud-clone corp., USA). The markers of oxidative stress, malondialdehyde (MDA) and total antioxidant capacity (TAC), were determined in cardiac tissue homogenates using MDA ELISA kit (Cloud-clone corp. USA), and OxiSelect™ TAC assay kit (Cell Biolabs, USA), respectively. The antioxidant capacity is determined relative to uric acid standards and therefore results were expressed as mM uric acid. Cardiac tissue homogenate was further used to assess inflammatory markers (TNF-α and IL-1β) and the apoptotic marker caspase-3 using their respective ELISA kits (Cloud-clone corp., USA).
Rat elisa kit
Manufactured by Cloud-Clone
Sourced in United States
Rat ELISA kits are laboratory tools used to detect and quantify specific proteins or other analytes in rat biological samples. These kits employ the enzyme-linked immunosorbent assay (ELISA) technique to provide accurate and reliable measurements. The core function of these kits is to facilitate the identification and analysis of target analytes in research applications involving rat models.
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Lab products found in correlation
5 protocols using rat elisa kit
1
Cardiac Biomarkers and Oxidative Stress
2
Quantification of Immunological Markers in Rats
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Blood was collected from the orbit of the rats on the 14th and 35th days for ELISA. According to the manufacturer’s instructions, myosin antibody (MYSAb), CXCL13 and IL‐21 were detected using Rat MYSAb ELISA Kit (OM626374; OmnimAbs, New Jersey, USA), Rat CXCL13 ELISA Kit (SEB601Ra; Cloud‐clone, China) and Rat ELISA Kit (SEB688Ra; Cloud‐clone, Hangzhou, China), respectively.
3
Quantifying LOX, MMP-2, and MMP-9 in Synovial Fluid
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LOX, MMP-2 and MMP-9 concentrations in the synovial fluid and serum were detected using rat ELISA kits (cat nos. SEC580Ra, SEA100Ra and SEA553Ra), according to the manufacturer's instructions (Cloud-Clone Corp., Houston, TX, USA). Synovial fluid was diluted at 1:10, and serum was diluted at 1:5. The optical density values were used to calculate the concentrations of LOX, MMP-2 and MMP-9 in all of the samples. The concentration was multiplied by the dilution fold in order to obtain the actual concentration.
4
Quantifying Aortic SIRT1 and SIRT3
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Aortic levels of SIRT1 and SIRT3 in homogenates (1:25) were measured using rat ELISA kits supplied by Cloud-Clone, Corp., Houston, TX, United States according to the manufacturer’s instructions.
5
Hepatic Cytokine and Protein Quantification
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Liver tissue homogenate (10%) was prepared in 0.05 M phosphate buffer (pH = 7) using a polytron homogenizer at 4 °C. The homogenate was centrifuged at 10,000 × g for 20 min for removing any cell debris. The supernatant was used after that for the determination of hepatic interleukin (IL)-1β, IL-6, and TGF-β1 levels using rat ELISA Kits (catalog # SEA563Ra, SEA079Ra, and SEA124Ra, respectively, Cloud-Clone Corp, USA). Hepatic contents of OCN and tumor necrosis factor-α (TNF-α) were determined using rat ELISA Kits (catalog # NBP2-68153, Novus Biologicals, and catalog # 438206, BioLegend Company, USA, respectively) following the manufacturer’s instructions (Wang et al. 2020 (link)) and (Guo et al. 2018 ).
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