Azathioprine

Manufactured by Selleck Chemicals

Azathioprine is a laboratory reagent used in chemical and biochemical research. It is a purine analog that can be used as a building block in the synthesis of various compounds. Azathioprine serves as a precursor for the production of other materials and is often employed in experiments related to cell biology, pharmacology, and drug development.

Automatically generated - may contain errors

Lab products found in correlation

3 protocols using azathioprine

Inhibition of Endocytic Pathways in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were treated with 10 μM rabeprazole (Rabe) or omeprazole (Ome) to inhibit PPs (Selleck, Shanghai, China). Cells were treated with 10 μM Cytochalasin D to inhibit phagocytosis (MedChemExpress, Shanghai, China). Cells were treated with 10 μM indomethacin (MedChemExpress) or chlorpromazine (Selleck) to inhibit caveolae‐ or clathrin‐mediated endocytosis, respectively. Macropinocytosis was inhibited by treatment cells with 10 μM LY294002 or amiloride (Selleck). Cells were treated with 10 μM KM91104 or 10 nM Baf‐A1, or 10 μM azathioprine (Selleck) to inhibit the activity of v‐ATPase or RAC1, respectively. Cells were treated with 10 μM Dynasore (MedChemExpress), CDC42‐IN‐1 (Selleck) or NAV‐2729 (MedChemExpress) to inhibit the activity of dynamin, CDC42 and ARF6, respectively. Cell cholesterol was extracted by treatment with 10 μM MeβCD (Aladdin). Equal pH gradients in cells were induced by 10 μM 2, 4‐DNP (Merck). Cells were treated with 10 μM H89 (Selleck) to inhibit PKA activation. In most experiments, cells were pretreated with the above reagents for 2 h, followed by subsequent experiments.

Lu X., Song Z., Hao J., Kong X., Yuan W., Shen Y., Zhang C., Yang J., Yu P., Qian Y., Zhang G., Feng H., Wang J., Cai Z, & Cai Z. (2024). Proton pump inhibitors enhance macropinocytosis‐mediated extracellular vesicle endocytosis by inducing membrane v‐ATPase assembly. Journal of Extracellular Vesicles, 13(4), e12426.

+ Open protocol

+ Expand

Reconstitution of Azathioprine, Trichostatin A, and Wortmannin

Check if the same lab product or an alternative is used in the 5 most similar protocols

Azathioprine was purchased from Selleckchem (http://www.selleckchem.com) in a powder form or in DMSO-dissolved form (catalog S1721). Trichostatin A (catalog S1045) and wortmannin (catalog S2758) were purchased from Selleckchem in a powder form.
Chemicals were reconstituted in 100% DMSO Hybri-Max™ (Sigma-Aldrich, catalog D2650) according to the manufacturer’s recommendations.

Reggio A., Spada F., Rosina M., Massacci G., Zuccotti A., Fuoco C., Gargioli C., Castagnoli L, & Cesareni G. (2019). The immunosuppressant drug azathioprine restrains adipogenesis of muscle Fibro/Adipogenic Progenitors from dystrophic mice by affecting AKT signaling. Scientific Reports, 9, 4360.

+ Open protocol

+ Expand

ARPE-19 and MDM Dengue Virus Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols

ARPE-19 or MDMs were seeded in six-well plates the day prior to infection and challenged with DENV at a multiplicity of infection (MOI) = 1 for ARPE-19 or MOI = 3 for MDMs, in serum-free medium. Cells were incubated with the inoculum for 90 min at 37°C in 5% CO₂ with rocking of the plates every 15 min. After incubation, the inoculum was removed, and cell monolayers washed once with phosphate-buffered saline (PBS, Gibco) then cultured in complete media. For experiments with drug treatment, cells were pre-treated for 2 h with 50 µM azathioprine (Selleck Chemicals) or 1 µM EHop-016 (Selleck Chemicals) prior to infection as above, with subsequent culture of the cells in the presence of drug. Uninfected or no drug treatment (vehicle, 0.01% dimethyl sulfoxide [DMSO]) controls were performed in parallel, and samples were collected at the indicated time point post-infection (pi) for analysis.

Cowell E., Jaber H., Kris L.P., Fitzgerald M.G., Sanders V.M., Norbury A.J., Eyre N.S, & Carr J.M. (2023). Vav proteins do not influence dengue virus replication but are associated with induction of phospho-ERK, IL-6, and viperin mRNA following DENV infection in vitro. Microbiology Spectrum, 12(1), e02391-23.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!