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Magnetic bead kits

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Magnetic bead kits are a type of laboratory equipment used for the separation and purification of various biological molecules, such as proteins, nucleic acids, and cells. The kits contain magnetic beads coated with specific ligands that can bind to the target molecules. When a sample is mixed with the magnetic beads, the target molecules are captured and can be separated from the rest of the sample using a magnetic field.

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Lab products found in correlation

Costar spin x centrifuge tube filter, by Corning (1 mentions) Spin x centrifuge filter unit, by Corning (1 mentions)

Spelling variants of this product

Magnetic beads (109 citations) Millipore multiplex kits with magnetic bead panels (3 citations) Multiplex MAP Magnetic Bead-based immunoassay kits (2 citations) Milliplex MAP bovine cytokine magnetic bead panel assay kits (2 citations) MILLIPLEX MAP rat Cytokine/Chemokine Magnetic Bead (MILLIPLEX MAP kits, (2 citations) MILLIPLEX MAP human circulating cancer biomarker magnetic bead panel kits (2 citations) Milliplex MAP Human Adipokine Magnetic Bead Panel 1 and Panel 2 kits (2 citations)

3 protocols using magnetic bead kits

1

Quantification of Vaginal Cytokines

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We quantified interferon‐gamma (IFNg), interleukin 1 beta (IL‐1b), interleukin 6 (IL6), interleukin 8 (IL8), interleukin 10 (IL10), and RANTES in vaginal fluid by adding 1 mL PBS to each thawed vaginal swab and agitating at low speed and room temperature for 2 hours. We filtered the sample using Costar® Spin‐X Centrifuge Tube Filter (Corning), tested the eluent in duplicate for cytokines using magnetic bead kits (EMD Millipore) according to manufacturer instructions, and analyzed with MAGPIX and xPONENT® 4.2 software (Luminex).
Achilles S.L., Meyn L.A., Mhlanga F.G., Matubu A.T., Stoner K.A., Beamer M.A., Chirenje Z.M, & Hillier S.L. (2020). Zim CHIC: A cohort study of immune changes in the female genital tract associated with initiation and use of contraceptives. American Journal of Reproductive Immunology, 84(3), e13287.
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2

Multiplex Immunoassay for Biological Fluid Analysis

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Plasma, serum, and the acellular synovial fluid fractions were placed in a warm bead bath until just thawed, then centrifuged at 1000× g for 10 min to remove debris. Approximately 300 µL of each sample were transferred to microcentrifuge tubes for multiplex immunoassays on the Luminex® 100/200TM System (Luminex Corporation, Tokyo, Japan, Cat #: LX200-XPON-RUO). Analytes were measured according to the manufacturer’s protocols using magnetic bead kits (all from EMD Millipore) described in Table 1. Resulting analyte concentrations were then calculated using the BelysaTM Immunoassay Curve Fitting Software System (EMD Millipore, Burlington, MA, USA, Cat #: 40-122).
Waltz R.A., Whitney K.E., Duke V.R., Kloser H., Huard C., Provencher M.T., Philippon M.J., Bahney C., Godin J.A, & Huard J. (2023). A Systemic and Local Comparison of Senescence in an Acute Anterior Cruciate Ligament Injury—A Pilot Case Series. Life, 13(7), 1567.
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3

Quantifying Cytokines and MMPs in Samples

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For cytokine and MMP analysis, each specimen was thawed at room temperature and the swab along with the specimen diluent, was centrifuged in a spin-X centrifuge filter unit (Costar, Cambridge, MA) at 12,000 rpm for 20 min. Aliquots of the filtered solution were stored at −80°C until analysis. Protein levels of cytokines and MMPs in the cell-free supernatants were measured (pg/mL) in duplicate through multiplexing with the use of magnetic bead kits (EMD Millipore, Billerica, Massachusetts, USA) according to the manufacturer’s instructions and analyzed on a MAGPIX (Luminex, Austin, Texas, USA) using the xPONENT® 4.2 software. Multiplexing allows for quantitative measurements of multiple analytes in a small volume of fluid (25 μL) and is based on attachment of the cytokines and MMPs to magnetic beads and processing using LED excitation. The minimum detectable concentrations (pg/mL for all analytes) defined by the manufacturer were: IL-1α = 9.4; IL-1β = 0.8; IL-4 = 4.5; IL-6 = 0.9; IL-8 = 0.4; IL-10 = 1.1; IL-13 = 1.3; TNF-α = 0.7; MMP-1 = 3; MMP-2 = 200; MMP-9 = 2; MMP-8 = 137. For values below the detection limit, we imputed the mid-point between 0 and the lowest threshold.
Caritis S.N., Hankins G., Hebert M., Haas D.M., Ahmed M., Simhan H., Haneline L.A., Harris J., Chang J., Famy A.S., Yorio P., Ren Z., D’Alton M.E, & Venkataramanan R. (2017). Impact of Pregnancy History and 17-hydroxyprogesterone caproate (17-OHPC) on Cervical Cytokines and Matrix Metalloproteinases. American journal of perinatology, 35(5), 470-480.
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