Hplc gradient grade acetonitrile

Tebuconazole (applied as internal standard) and formic acid were purchased from the Merck-Sigma group (St. Louis, MO, USA). HPLC gradient grade acetonitrile, methanol, and HPLC grade ethanol were ordered from Fisher Scientific (VWR, Radnor, PA, USA). Ultra-pure water (> 18 MΩ• cm) was obtained from a Merck-Millipore Milli-Q system (Bedford, USA). Peloruside A authentic standard material was gained by chemical synthesis and also by isolation from Mycale hentscheli marine sponge samples with the kind contribution of Dan Sackett (Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, USA) and Peter Northcote (Victoria University of Wellington, Wellington, New Zealand) and their workgroups, respectively. Peloruside A stocks were prepared in ethanol and were stored at − 18 °C until analysis.

Formic acid, trifluoroacetic acid, ammonium formate (10 M), ammonium bicarbonate TCEP (Tris (2-carboxyethyl)phosphine hydrochloride), MMTS (Methyl methanethiosulfonate) and trypsin were all obtained from Sigma. HPLC gradient grade acetonitrile was obtained from Fisher Scientific. Briefly, 2 × 10⁶ hTERT-BJ1 fibroblasts were seeded in 150 cm plates until cells were attached. Cells were then treated with azathioprine or taxol at the concentrations indicated. As control, vehicle-treated cells were processed in parallel. After 48 hours of treatment, cells were lysed in RIPA buffer (R0278, Sigma) and kept at 4°C for 20 minutes with rotation. Lysates were cleared by centrifugation for 10 minutes at 10,000 × g and supernatants were collected and kept frozen at −80°C.

Formic acid, trifluoroacetic acid, ammonium formate (10 M), ammonium bicarbonate TCEP (Tris (2-carboxyethyl)phosphine hydrochloride), MMTS (Methyl methanethiosulfonate) and trypsin were all obtained from Sigma. HPLC gradient grade acetonitrile was obtained from Fisher Scientific. Briefly, 2 × 10⁶ MCF7 cells were seeded in 15 cm plates until cells were attached. Cells were then treated with 10 µM mDIVI1. As control, vehicle-treated cells were processed in parallel. After 48 hours of treatment, cells were lysed in RIPA buffer (R0278, Sigma) and kept at 4° C for 20 minutes with rotation. Lysates were cleared by centrifugation for 10 minutes at 10,000 × g and supernatants were collected and kept frozen at −80° C.

Acetonitrile (gradient HPLC grade) was obtained from Fischer Scientific (Hampton, NH, USA). Ultrapure water was obtained with a Milli-Q water system (Millipore Merck, Darmstadt, Germany). Acetone (≥99.8%), n-hexane (≥95%), HCl (37%) were obtained from Honeywell (Charlotte, NC, USA), NaOH (p.a.), NH₃ (25%), acetic acid (glacial), NaH₂PO₄ × 2H₂O (p.a.) and NaHCO₃ (p.a.) from Merck (Darmstadt, Germany). Dansyl chloride (≥99%) and amines were obtained from Sigma-Aldrich (St. Louis, MO, USA): 1,7-diaminoheptane (98%), agmatine sulfate (≥97%), phenethylamine (99%), histamine (≥97%), cadaverine (≥96.5%), putrescine (≥98.5%), spermidine (≥98%), spermine (≥97%), tyramine (≥98.5%) and tryptamine (TRP) (≥98%).
Fenugreek (Trigonella foenum-graecum), lentil (Lens esculentum), alfalfa (Medicago sativa), and daikon radish (Raphanus sativus) seeds designated for sprouting were supplied by Amarant (Kresnice, Slovenia).

Acetonitrile (gradient HPLC grade) was obtained from Fischer Scientific (Hampton, NH, USA). Ultrapure water was obtained with a Milli-Q water system (Millipore Merck, Darmstadt, Germany). Acetone (≥99.8%), n-hexane (≥95%), and HCl (37%) were obtained from Honeywell (Charlotte, NC, USA), NaOH (p.a.); NH₃ (25%), acetic acid (glacial), NaH₂PO₄ × 2H₂O (p.a.), and NaHCO₃ (p.a.) were obtained from Merck (Darmstadt, Germany). Trolox, Folin–Ciocalteu reagent, dansyl chloride (≥99%), GABA (≥99%), and amines: 1,7-diaminoheptane (98%), phenethylamine (99%), histamine (≥97%), cadaverine (≥96.5%), putrescine (≥98.5%), spermidine (≥98%), spermine (≥97%), tyramine (≥98.5%), and tryptamine (≥98%) were obtained from Sigma-Aldrich (St. Louis, MO, USA).
Three wholemeal chickpea flours (Flour-1, Flour-2, Flour-3) with a certificate of organic origin from different suppliers were obtained in specialized shops for organic products in Ljubljana, Slovenia. All experiments with flours were performed prior to the best-before date marked on the packaging. Commercial starter culture Livendo^TM starter LV1 was obtained from Lesaffre (Lille, France).

Polylactic acid (PLA L207S) was purchased from Evonik, Germany. A pharmaceutical grade birch bark triterpene extract from the outer bark of birch (TE) was obtained from Amryt AG, Niefern-Öschelbronn, Germany, comprising betulin 81.60%, lupeol 2.08%, betulinic acid 3.84%, erythrodiol 1.05%, oleanolic acid 0.97%, betulinic acid methyl ester 0.52%, and undisclosed substances 9.94%. betulin was supplied by Sigma-Aldrich. Dimethyl sulfoxide (DMSO, laboratory reagent grade), dichloromethane (DCM, HPLC grade), and acetonitrile (HPLC gradient grade) were purchased from Fisher Scientific, UK.