TPP and its metal complexes of Pd(II)TPP and Pt(II)TPP were synthesized in the laboratory. All chemicals were used as received without any further purification. FTIR spectra were recorded on a Bruker Vertex 70 spectrometer. XPS was performed using an SKL-12 spectrometer modified with a VG CLAM 4 multichannel hemispherical analyzer. Photo-luminescence (PL) spectra were recorded on a Perkin-Elmer LS 55 spectrofluorometer. The electronic absorption spectra in the ultraviolet/visible (UV/vis) region were recorded with a Hewlett Packard 8453 UV/vis spectrophotometer. TEM analyses were performed on an FEI-Tecnai G2 S-Twin TEM, operating at an acceleration voltage of 200 kV. X-ray powder diffraction measurements were investigated using a Bruker D8 Advance diffractometer through nonmonochromatic Cu Kα X-ray at 40 kV, 40 mA.
8453 uv vis spectrophotometer
Manufactured by Hewlett-Packard
Sourced in United States
The Hewlett-Packard 8453 UV/vis spectrophotometer is a laboratory instrument designed to measure the absorbance or transmittance of light in the ultraviolet and visible regions of the electromagnetic spectrum. It is capable of analyzing the composition and concentration of various samples by detecting the amount of light that is absorbed or transmitted through the sample.
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Lab products found in correlation
Vertex 70 spectrometer, by Bruker (1 mentions)
Vertex 70 ftir spectrometer, by Bruker (1 mentions)
D8 discover xrd system, by Bruker (1 mentions)
D8 advance 800234 x ray, by Bruker (1 mentions)
Axis ultra spectrometer, by Shimadzu (1 mentions)
Senterra raman spectrometer, by Bruker (1 mentions)
Ultima 4 xrd, by Rigaku (1 mentions)
H 7650 transmission electron microscope, by Hitachi (1 mentions)
Anhydrous dioxane, by Merck Group (1 mentions)
400 mhz spectrometer, by Bruker (1 mentions)
Smart apex 2 ccd diffractometer, by Bruker (1 mentions)
C11347 quantaurus qy integrating sphere, by Hamamatsu Photonics (1 mentions)
Tga q5000, by TA Instruments (1 mentions)
1525 hplc, by Waters Corporation (1 mentions)
Fls980 fluorescence spectrophotometer, by Edinburgh Instruments (1 mentions)
Vector 22 spectrometer, by Bruker (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Saturn diffractometer, by Rigaku (1 mentions)
Av 400 spectrometer, by Bruker (1 mentions)
12 protocols using 8453 uv vis spectrophotometer
1
Synthesis and Characterization of TPP and Metal Complexes
2
Steady-State and Time-Resolved Spectroscopy of 2,2-Diphenyl-2H-chromene
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For all measurements samples were prepared under an inert atmosphere of dry nitrogen. Deuterated acetonitrile (CD3CN, purchased from Euriso-top) was used as solvent because of its favourable absorption characteristics in the 1400–1800 cm−1 spectral region. The steady-state UV/vis spectrum of 2,2-diphenyl-2H-chromene (Fig. 3 ) was recorded using a Hewlett Packard 8453 UV/vis spectrophotometer. The steady-state IR absorption spectrum of the compound, shown in Fig. 4 , was obtained using a Bruker Vertex 70 FTIR spectrometer. For the time-resolved measurement samples were prepared in the same manner as described above, but with a concentration adjusted to give an absorbance of 0.8 at 320 nm in a sealed IR flow cell equipped with UV-grade CaF2 windows with a 1 mm thick spacing (Specac).
3
Structural Characterization of Quercetin-PABA Nanoparticles
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The structure of quercetin – para-aminobenzoic acid (QPABA) was determined using both 1H and 13C NMR Bruker 600 MHz operated through Topspin 3.0 NMR software. UV-vis spectra of the resulting QPABA-based nanoparticle solutions were determined using Hewlett-Packard 8453 UV-vis spectrophotometer from 200 to 1000 nm. The samples for TEM analysis were prepped via drop-casting the samples of liquid aliquots on SiO2-coated 300 mesh copper grids. The samples are dried thereafter. The nanoflowers' size and morphology were examined and analyzed using Axion Vision software version 4.8.2. TEM. A drop of the sample was added to the TEM grid and dried at room temperature for TEM imaging. The experiment was performed, and the images were recorded. The TEM images and size distribution measurements of AuNFs and AuNS were prepared. The XRD measurements for the samples were analyzed using a Bruker D8 Discover XRD system operating at 40 kV and 40 mA. The XRD samples were dissolved in ethanol and then mounted on sample holders for measurements. Furthermore, XRD patterns were obtained using an X-ray diffractometer (Bruker D8 Advance 800234-X-ray (9729), Germany) using Cu-Kα radiation (l ¼ 1.5178 A, 40 kV, and 40 mA). The 2-theta angle scanning range was from 5 to 80 with a scanning speed of 0.1 per second.
4
Characterization of CdSQDs@pNIPAm-co-AAc Microgels
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UV-vis absorption spectra of CdSQDs@pNIPAm-co-AAc hybrid microgels were recorded on a Hewlett Packard 8453 UV-vis Spectrophotometer (USA). The size and morphology of CdSQDs@pNIPAm-co-AAc hybrid microgels were determined at 60 to 120 kV accelerating voltage by a Hitachi H-7650 transmission electron microscope (TEM, Japan), and the electron diffraction pattern for the composite microgels was also measured. X-ray photoelectron spectroscopy (XPS) was performed on a Kratos AXIS Ultra spectrometer equipped with a monochromated Al Kα (hν = 1486.6 eV) X-ray source (Kratos Analytical, UK). A Rigaku XRD Ultima IV (Japan) using Cu Kα X-ray source (40 kV, 44 mA) and continuous scan mode was used to collect data, which was examined using JADE software. Samples for Raman spectroscopy were placed onto 2 cm2 glass slides. Measurements were conducted using a Senterra Raman spectrometer (Bruker, USA) equipped with a laser diode (λ = 785 nm) of 10 mW laser power. Mass spectrometry was used to analyze the photodegradation products of RhB using a Voyager Elite MALDI-TOF mass spectrometer (AB Sciex, Canada) equipped with a pulsed nitrogen laser (337 nm, 3 ns pulse).
5
Anhydrous Solvent Handling and NMR Analysis
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All air- and moisture-sensitive reactions were conducted under a dry nitrogen atmosphere in oven-dried glassware, using standard syringe/cannula transfer techniques unless otherwise reported. Anhydrous dioxane (Sigma Aldrich) was used directly from a Sure/Seal bottle. Unless otherwise stated, all commercial reagents were purchased from Sigma Aldrich and used without further purification. 1H NMR spectra were recorded on a Bruker 400 MHz spectrometer in either CDCl3 or DMSO-d6. Chemical shifts (δ) are reported in parts per million (ppm) after calibration to the internal reference solvent peak (CDCl3: 7.26 ppm and DMSO-d6: 2.50 ppm). Coupling constants (J) are reported in Hz. Exact mass was calculated for M+Na using electrospray ionization unless reported otherwise. Using a Hewlett Packard 8453 UV-Vis spectrophotometer, solubility was obtained either by measuring the increase in light scatter upon increasing precipitation via absorbance at 700 nm, or by using absorbance calibration curves at the noted wavelength and then extrapolating concentration from saturated sample absorbance [33 (link)].
6
UV-Vis Spectroscopy of Quartz Micro-Cuvettes
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UV-visible spectra were recorded using a Hewlett Packard 8453 UV-Vis spectrophotometer (Hewlett Packard, Palo Alto, CA, USA). Measurements were performed using a quartz micro-cuvette (1 mm × 1 cm) in the wavelength range of 250 nm to 800 nm with 1 nm resolution at room temperature.
7
Characterization of Organic Compounds
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1H NMR and 13C NMR spectra were measured on a Bruker AV 400 spectrometer at 298 K. Absorption spectra were recorded on a Hewlett Packard 8453 UV–Vis spectrophotometer. High-resolution mass spectra (HRMS) were obtained on a GCT Premier CAB 048 mass spectrometer operated in MALDI-TOF mode. Fluorescent emission spectra were collected on a Horiba FluoroLog 3 fluorometer at 298 K. Solid state quantum yield was measured using a Hamamatsu C11347 Quantaurus-QY integrating sphere. High performance liquid chromatography (HPLC) was performed on an Waters 1525 HPLC. The running rate was 10 mL/min, and running buffer was 70% methanol and 30% water. The lifetime, steady state and low temperature photoluminescence spectra were measured on a Edinburgh FLS980 fluorescence spectrophotometer equipped with a continuous xenon lamp (Xe1), a microsecond pulsed xenon flash lamp (uF920) and a nanosecond flash lamp (nF920), respectively. Single crystal data were collected on a Bruker Smart APEXII CCD diffractometer using graphite monochromated Mo Kα radiation (λ = 0.71073 Å) or Cu Kα radiation (λ = 1.54184 Å). The thermogravimetric analysis (TGA) measurements were performed on a TA Instrument TGA Q5000.
8
Spectroscopic Analysis of Carbohydrates
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Ultraviolet–visible (UV–vis) spectra were acquired according to a Hewlett-Packard 8453 UV–vis spectrophotometer. Optical rotations were measured with an A22109 Autopol Manual Revision C Rudolph polarimeter (Rudolph, Hackettstown, NJ, USA). Fourier transform infrared (FT-IR) spectra of KBr pellets were obtained using a Bruker Vector-22 spectrometer. Using the phenolsulfuric acid method, the total carbohydrate content was calculated as D-glucose equivalents [38 (link)]. Uronic acid content was determined using an m-hydroxydiphenyl colorimetric method that does not interfere with neutral sugars [39 (link)].
9
Standardized Hemolysis Assay Protocol
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For the standard hemolysis assessment, a volume of 0.2 mL of freshly obtained blood from the lateral tail vein was collected into vacutainers containing heparin. The blood sample was then combined with sterile physiological serum at a ratio of 4:5. As part of the experimental setup, sterile physiological serum served as a negative control, and Triton X-100 (catalogue code X-100, Sigma-Aldrich Chemical Co, Steinheim, Germany) at 10% (v/v), an agent known for its hemolytic activity, was used as a positive control [70 (link)]. The mixtures were put to incubate for 45 min at 37 °C. Following this incubation period, 0.2 mL of extraction medium from each sample was placed in a water bath at 37 °C for one hour. Subsequently, each tube underwent centrifugation for 10 min at 1000 RCF (relative centrifugal force). The absorbance of the resulting supernatant was measured at a wavelength of 545 nm using a Hewlett Packard 8453 UV–VIS spectrophotometer (Waldbronn, Germany).
The hemolysis ratio (hemolysis %) was determined as opposed to a completely hemolyzed solution, employing the following formula [71 (link)]:
The hemolysis ratio (hemolysis %) was determined as opposed to a completely hemolyzed solution, employing the following formula [71 (link)]:
10
Zn(OTf)2 interaction with PUU-Py
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UV-vis spectroscopy was carried out using Hewlett Packard 8453 UV-vis Spectrophotometer (G1103A), where 17 μL of Zn(OTf)2 solution in methanol was each time added to 2 mL of PUU-Py solution in THF (0.25 mg/mL). UV-vis spectroscopy of PUU-HMD was carried out by its solution in DMF because of relative good solubility compared to in THF.
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