2 amino 2 hydroxymethyl 1 3 propanediol trizma base

PEG tetra-vinyl sulfone (10 kDa, PEG-VS) and PEG tetra-thiol (10 kDa, PEG-SH) purchased from JenKem Technology, USA. Oxygen-sensitive phosphor Pd(II) meso-tetra(sulfophenyl) tetrabenzoporphyrin sodium salt (S-PdBP) was purchased from Frontier Specialty Chemicals, USA. Glucose oxidase (≤ 40 U/mg) from Aspergillus niger, (Z,Z,Z)-Sorbitan tri-9-octadecenoate (SPAN 85), and polyoxyethylenesorbitan trioleate (TWEEN 85) were purchased from Tokyo Chemical Industry, USA. Catalase from bovine liver (2,000-5,000 U/mg), alginic acid sodium salt from brown algae (viscosity of ~250 cps at 2% solution, average Mw 75,000-100,000 Da), poly(allylamine hydrochloride) (PAH) (average Mw 17,500 Da), poly(Sodium 4-styrenesulfonate) (PSS) (average Mw ~70,000 Da), 2-amino-2-(hydroxymethyl)-1,3-propanediol (TRIZMA base), triethanolamine (TEOA), 2-(N-morpholino) ethane-sulfonic acid sodium salt (MES sodium salt), and calcium carbonate were purchased from Sigma Aldrich Chemical Co., St. Louis, MO, USA. Isooctane, dimethyl sulfoxide (DMSO), tris(hydroxymethyl) aminomethane (TRIS) hydrochloride, and phosphate buffered saline (PBS) tablets were purchased from Avantor Performance Materials, LLC, USA. Calcium chloride dihydrate was purchased from VWR Chemicals, LLC, OH, USA. All reagents were used as received without further purification unless mentioned otherwise.

Dynabeads® MyOne™ Streptavidin C1 paramagnetic beads were purchased from Invitrogen (Carlsbad, CA). Salmonella enterica, serovar typhimurium LT2 DNA (700720D-5) was purchased from ATCC (Manassas, VA). Biotinylated oligonucleotides were purchased from Eurofins MWG Operon (Huntsville, AL). Potassium chloride and ethanol were purchased from Fisher Scientific (Fair Lawn, NJ). 2-Amino-2-(hydroxymethyl)-1,3-propanediol (Trizma base, 99.9%) was purchased from Sigma (St Louis, MO). All solutions were prepared in Nanopure water (Barnstead/Thermolyne, Dubuque, IA). SpeedSTAR™ polymerase, magnesium chloride and 10× PCR buffer, is available from Clontech Lab. (Mountain view, CA).