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2 protocols using 6 gingerol

1

Simultaneous Quantification of Phytochemicals

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A 0.5 gm powdered Divya-Swasari-Vati sample was diluted with 10 mL of methanol:water (80:20) and sonicated for 30 minutes, centrifuged at 10,000 rpm for 5 minutes, and filtered using 0.45 µm nylon filter. The standard compounds: Gallic acid was purchased from Loba Chemie (Mumbai, Maharashtra, India); Ellagic acid, Piperine, Eugenol, Glycyrrhizin, Coumarin from Sigma-Aldrich (St. Louis, MO, USA); Protocatechuic acid, Glabridin from Natural Remedies (Greater Noida, Uttar Pradesh, India), Methyl gallate from Tokyo Chemical Industry (Tokyo, Japan), 6-Gingerol from Cayman Chemical (Ann Arbor, MI, USA), and Cinnamic acid from Sisco Research Laboratories (Mumbai, Maharashtra, India). Standards were dissolved in methanol to prepare the appropriate concentrations.
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2

Comprehensive Chemical Analysis of Divya-Swasari-Vati

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The AR grade solvents, toluene, ethyl acetate, formic acid, acetic acid and methanol (HPLC grade) were procured from Merck (Darmstad, Germany), acetonitrile from Honeywell (Dusseldorf, Germany) and deionized water was obtained from a Milli Q system (Millipore, Billerica, MA, USA). Authentic standards of gallic acid (Cat No. 91215, Sigma Aldrich, St. Louis, MO, USA), protocatechuic acid (Cat No. P006, Natural Remedies, Bangalore, Karnataka, India), coumarin (Cat No. C4261, Sigma Aldrich, St. Louis, MO, USA), cinnamic acid (Cat No. 29955, Sisco Research Lab, Mumbai, Maharashtra, India), eugenol (Cat No. 35995, Sigma Aldrich, St. Louis, MO, USA), 6-gingerol (Cat No. 11707, Cayman Chemical, Ann Arbor, MI, USA), piperine (Cat No. P49007-5G, Sigma Aldrich, St. Louis, MO, USA), glabridin (Cat No. G005, Natural Remedies, Bangalore, Karnataka, India), ellagic acid (Cat No. E2250, Cayman Chemical, Ann Arbor, MI, USA) and glycyrrhizin (Cat No. G008, Natural Remedies, Bangalore, Karnataka, India) were used for the analysis. Samples from five different batches of Divya-Swasari-Vati, (#B SWV117, #B SWV084, #A SWV023, #A SWV102 and #B SWV239) were used for the chemical analysis. DSV samples were sourced from Divya Pharmacy (Haridwar, India) and were stored in airtight bottles for further use.
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