Miracle atr device

RC-33 was synthesized as described in [13 (link)]. Herein, the characterization of RC-33 HCl was reported. Rf = 0.40 (TLC: 8/2 AcOEt/MeOH, v/v). mp 2088–2094 °C; IR (cm⁻¹) 3053, 2948, 2605, 2478, 1487, 1450, 1441, 1401, 1065, 1008, 959, 836, 766, 735; ¹H NMR (D₂O) δ (ppm): 12.10 (brs, 1H, H salt) 7.61 (m, 4H, Ar); 7.45 (m, 2H, Ar); 7.32 (m, 3H, Ar); 3.35 (m, 2H, ArCHCH₂CH₂); 2.97 (m, 1H, ArCH); 2.73 (m, 4H, Pip); 1.96 (m, 2H, ArCHCH₂); 1.78 (m, 2H, Pip); 1.69 (m, 1H, Pip), 1,32 (m, 2H, Pip); 1.24 (m, 1H, Pip); 1.22 (d, 3H, ArCHCH₃).
Melting points were measured on SMP3 Stuart Scientific apparatus and are uncorrected. For FT-IR analysis a Spectrum One Perkin Elmer spectrophotometer equipped with a MIRacle™ ATR device was used.

The physical-chemical properties of all the freeze-dried products have been investigated as reported in [7 (link)]. In detail, the IR spectra in transmittance mode were obtained by a Spectrum One Perkin-Elmer spectrophotometer equipped with a MIRacle™ ATR device in the spectral region of 650–4000 cm⁻¹. Differential Scanning Calorimetry (DSC) analysis was performed by Mettler STARe system equipped with a DSC821e Module and an Intracooler device (Julabo FT 900) for sub-ambient temperature. The residual humidity was investigated by Thermo Gravimetric Analysis (TGA) with a Mettler STARe system equipped with a TGA/DSC1.

FT-IR analysis was performed to confirm the simultaneous presence of both soluble proteins and EVs into lyo-secretome. FT-IR spectra were collected using a Spectrum One Perkin-Elmer spectrophotometer equipped with a MIRacle™ ATR device. The IR spectra in transmittance mode were obtained in the spectral region of 650–4000 cm⁻¹ by the accumulation of 64 scans with a resolution of 4 cm⁻¹.