In serum, glucose concentration was measured by Contour glucose monitor (Bayer, IN, USA) and corticosterone was measured by a high sensitive-corticoid kit (ADI-900, Enzo, NY, USA) according to manufacturer’s instructions.
Contour glucose monitor
Manufactured by Bayer
Sourced in Germany
The Contour glucose monitor is a device used to measure blood glucose levels. It provides a quick and convenient way for individuals to monitor their glucose levels. The device requires a small blood sample and displays the result on its digital screen.
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7 protocols using contour glucose monitor
1
Serum glucose and corticosterone analysis
2
Intraperitoneal Glucose Tolerance Test
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After 6 h fasting with ad libitum water, mice were intraperitoneally injected with 2 g/kg body wt d -glucose. Glucose concentration was measured in blood obtained from tail tip at 0, 15, 30, 60, and 120 min after administration using a Contour glucose monitor (Bayer).
3
Oral Insulin Tolerance Test in Mice
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After 10 weeks of EGCG treatment, mice underwent an oral ITT (40 (link)). Mice were deprived of food for 4 h, and the ITT was performed after intraperitoneal injection of insulin (0.75 UI/kg). Blood glucose concentrations were measured at 0 min, and then at 15, 30, 60, 90 and 120 min after insulin injection using a portable Contour® glucose monitor (Bayer AG) and test strips. The concentration of insulin C-peptide in blood serum at 120 min was measured using a mouse insulin C-peptide radioimmunoassay kit (cat. no. DIC P00; R&D Systems, Inc.), according to the manufacturers protocols.
4
Glucose and Insulin Homeostasis Assessment
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Blood glucose and Serum insulin concentrations were measured in male mice of indicated age after a 6 to 12 AM 6 hr fast (ip. glucose tolerance test) or at random fed state at 2 PM (ip. insulin tolerance test) as described [8 (link),11 (link),12 (link)]. Blood glucose was measured before and after intraperitoneal injections of glucose (2g/kg or 1 g/kg) or insulin (1 U/kg Actrapid HM, Novo Nordisk, Denmark) with a Contour glucose monitor (Bayer Healthcare, Germany) by tail vein sampling at indicated time points in duplicates. The upper detection limit of the glucose monitor used was a glucose concentration of 33.3 mmol/L, values exceeding this limit were counted as 33.3 mmol/l. Serum insulin (CrystalChem, Downers Grove, IL, USA), serum leptin (CrystalChem), serum adiponectin (CrystalChem) and plasma glucagon (Mercodia, Uppsala, Sweden) concentrations were determined by ELISAs.
5
Glucose and Insulin Dynamics in Fasted Mice
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Experiments were performed in the early afternoon on mice starved for 6 h (Andrikopoulos et al., 2008 (link)). Blood glucose was measured before and after intraperitoneal injections of glucose (2 g/kg) or insulin (1 U/kg Actrapid HM, Novo Nordisk, Denmark) using a glucometer (Contour glucose monitor, Bayer Healthcare, Germany) after tail vein sampling at indicated time points. Serum insulin concentrations were determined by ELISAs (CrystalChem, Downers Grove, IL, United States).
6
Glucose and Insulin Tolerance Tests in Mice
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Tolerance tests were performed in 10-12 week-old male mice after a 6 am to 12 pm 6-hour fast (intraperitoneal glucose tolerance test [IPGTT]) or at random fed state at 2 pm (intraperitoneal insulin tolerance test [IPITT]), as described. 21 (link) , 22 (link) Blood glucose was measured at time-30, 0, 15, 30, 60, and 120 minutes with a Contour glucose monitor (Bayer Healthcare, Germany) by tail vein sampling in duplicates. Vehicle or hydrochlorothiazide (HCT) was applied by intraperitoneal injection at time -30 minutes. Glucose (IPGTT; 1 or 2 g/kg, Sigma-Aldrich) or insulin (IPITT; 0.5 or 1 U/kg Actrapid HM, Novo Nordisk, Denmark) were applied by intraperitoneal injection at time 0 minute. For serum insulin measurements, tail vein blood sampling was performed at time -30, 0, and 2 minutes. Vehicle or HCT was applied at time -30 minutes and glucose (2 g/kg) was applied at time 0 minute. Serum insulin was measured with the ultrasensitive mouse insulin ELISA (CrystalChem, Downers Grove, IL; #90080).
7
Fasting Glucose and Insulin Resistance Evaluation
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