2-week-old male Sel1LAlb mice were injected i.p. with a single dose of 25 mg/kg diethylnitrosamine (DEN; Sigma N0258), then put on high fat diet at 4 weeks of age, and finally euthanized for analysis at 28 weeks of age.
N0258
Manufactured by Merck Group
The N0258 is a laboratory equipment product manufactured by Merck Group. It is designed for general laboratory applications. The core function of the N0258 is to facilitate standard laboratory procedures and processes.
Automatically generated - may contain errors
9 protocols using n0258
1
DEN-induced Liver Cancer Model in Sel1L Mice
2
Liver Cancer Model in Conditional Knockout Mice
3
Diethylnitrosamine-induced Hepatocellular Carcinoma Mouse Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Diethylnitrosamine (DEN)‐induced HCC mouse model was established as described previously.[70 (link)
] In brief, 2‐week‐old mice were injected with DEN (20 mg k−1g body weight, Sigma, N0258) to start the growth of tumors. The phenobarbital‐like inducer TCPOBOP (3 mg k−1g body weight; ApexBio, B8576) was injected every 2 weeks (a total of 8 times) to accelerate HCC progression after DEN injection for 2 weeks. All mice were kept in a controlled environment (23–25 °C with a 12‐h light‐dark cycle and lights on at 8:00 AM), on normal diet feeding with free access to water. For FigureS8g–k of the Supporting Information to examine liver inflammation and fibrosis, all data were collected from mice at 9:00 AM. For the other HCC mouse model, mice were fasted for 12 h before bleeding and sacrifice at 9:00 AM.
] In brief, 2‐week‐old mice were injected with DEN (20 mg k−1g body weight, Sigma, N0258) to start the growth of tumors. The phenobarbital‐like inducer TCPOBOP (3 mg k−1g body weight; ApexBio, B8576) was injected every 2 weeks (a total of 8 times) to accelerate HCC progression after DEN injection for 2 weeks. All mice were kept in a controlled environment (23–25 °C with a 12‐h light‐dark cycle and lights on at 8:00 AM), on normal diet feeding with free access to water. For Figure
4
Liver Tumor Induction in Mice
5
Hepatocarcinogenesis Model in C3H/HeOuJ Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C3H/HeOuJ male and female mice were purchased from Jackson Laboratory (Bar Harbor, ME), and maintained on a light: dark 12:12 schedule. ZT24/0 is the time of light-on and ZT12 is the time of light-off. Animal studies were approved by the University of North Carolina School of Medicine (Institutional Animal Care and Use Committee).
P15 male C3H/HeOuJ mice were treated with a single intraperitoneal (IP) injection of DEN (Sigma-Aldrich N0258; 20 mg/kg body weight) diluted in 0.85% saline to stimulate hepatocarcinogenesis. Livers from healthy control (−DEN) mice and +DEN mice were collected 25 weeks after treatment at the earliest. Tumors were resected from livers of DEN-treated mice for analysis. Males were used since they reliably develop liver tumors; females do not efficiently biotransform DEN to the ultimate carcinogen and are not suitable for use (44 (link)).
P15 male C3H/HeOuJ mice were treated with a single intraperitoneal (IP) injection of DEN (Sigma-Aldrich N0258; 20 mg/kg body weight) diluted in 0.85% saline to stimulate hepatocarcinogenesis. Livers from healthy control (−DEN) mice and +DEN mice were collected 25 weeks after treatment at the earliest. Tumors were resected from livers of DEN-treated mice for analysis. Males were used since they reliably develop liver tumors; females do not efficiently biotransform DEN to the ultimate carcinogen and are not suitable for use (44 (link)).
6
Establishment of NAFLD-HCC Mouse Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Two weeks old C57BL/6 mice were purchased from Charles River (Beijing, China), and fed with HFD (MD12032, Medicinece, Jiangsu, China) for 16 weeks to make the NAFLD model. Subsequently, diethylnitrosamine (DEN, N0258, Sigma, St. Louis, MO) with 45 mg/kg i.p. in PBS was injected into NAFLD mouse model via intraperitoneal. All liver tissues were separated at 8, 16 and 36 weeks later for HFD feeding and 20 weeks later for DEN treatment, separately. The animal studies were approved by the Institutional Animal Care and Use Committee of the Second Affiliated Hospital of Nanchang University.
7
Hepatocellular Carcinoma Mouse Models: Induction and Recurrence
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A diethylnitrosamine (DEN)-induced HCC model was constructed by administering a single intraperitoneal injection of DEN (Sigma-Aldrich, # N0258) to 14-day-old male mice. Eight months later, tumours were harvested and cut into 2 × 2 mm pieces, then the fresh tumour tissues were subcutaneously injected into the right flanks of WT mice to stabilise the tumours. When tumours reached 1 × 1 cm, they were cut into 1 × 1 mm fragments and directly injected into the livers of WT mice. Other HCC models were constructed by administering a single intrahepatic injection of Hepa1–6 cells (1·5 × 10 [6 (link)]/20 μl) or a single intra-splenic injection of Hepa1–6 cells (1·5 × 10 [6 (link)]/50 μl or 2·0 × 10 [6 (link)]/50 μl) into male C57BL/6 mice. To study recurrence, tumours were observed two weeks later through the opened abdominal cavity. Then, tumours were resected with an electrosurgical knife, followed by intraperitoneal injection of phosphate-buffered saline (PBS) (controls) or napabucasin (40 mg/kg) twice per week. Finally, all mice were sacrificed at the specified time points.
8
Liver Tumorigenesis in C3H Mouse Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Animal experimentation was carried out in accordance with the Animals (Scientific Procedures) Act 1986 (United Kingdom) and with the approval of the Cancer Research UK Cambridge Institute Animal Welfare and Ethical Review Body. Inbred female M. musculus castaneus (CAST/EiJ) mice were crossed with inbred male C3H/HeOuJ (C3H) mice. The F1 male offspring were treated with a single intraperitoneal dose of DEN (Sigma-Aldrich N0258; 20 mg kg−1 body weight) as described previously14 (link). Liver tumors were isolated 30 weeks after treatment, flash frozen in liquid nitrogen and stored at −80 °C for DNA extraction and sequencing. Liver tissue from an untreated P15 litter mate was sampled for control experiments. Control samples (liver tissue) were also collected from untreated, age-matched littermates. Animals were maintained using standard husbandry: mice were group housed at room temperature/humidity in Tecniplast GM500 IVC cages with a 12 h:12 h light:dark cycle and ad libitum access to water, food (LabDiet 5058), and environmental enrichments.
9
Liver Cancer Induction in Mice
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!