Es1000w digital camera

MCF-7 cells grown on coverslips in 24-well plates (BD Falcon) were incubated with 0.25 mg ml⁻¹ SPION for different times. Samples were washed with PBS and fixed with a mixture of 2 % paraformaldehyde (Polysciences Inc.) and 2.5 % glutaraldehyde (TAAB Laboratories) in PBS (1 h, room temperature). The cell monolayer was washed with PBS and distilled water, post-fixed (45 min) with 1 % osmium tetroxide in PBS (TAAB Laboratories), washed with distilled water, treated (45 min) with 1 % aqueous uranyl acetate (Electron Microscopy Sciences), dehydrated with increasing concentrations of ethanol (SeccoSolv; Merck) and embedded in epoxy resin EML-812 (TAAB Laboratories; 2 day, room temperature). Resin-containing gelatin capsules (TAAB) were placed on coverslips and polymerised (2 days, 60 °C). Resin blocks were detached from coverslips by successive immersion in liquid nitrogen and hot water. Ultrathin 70 nm-thick sections were obtained with the Ultracut UCT ultramicrotome (Leica Microsystems), transferred to 200 mesh nickel EM grids (Gilder) and stained with 3 % aqueous uranyl acetate (20 min) and lead citrate (2 min). Sections were visualised on a JEOL JEM 1200 EXII electron microscope (operating at 100 kV). Micrographs were taken with a Gatan Erlangshen ES 1000 W digital camera at various magnifications.

Quadriceps muscles from Ndufs3 Mlc1f‐KO and wild type of 8 months old were fixed in 2.5% glutaraldehyde overnight and treated with 1% osmium tetroxide, dehydrated in ethanol, and embedded in epoxy resin. Semi‐thin (1 μm) sections were stained with Richardson's stain. For transmission electron microscopy (TEM), ultra‐thin sections (100 nm) were cut on grids, stained with uranyl acetate, and lead citrate and scope in a Philips CM‐10 transmission electron microscope equipped with a Gatan Erlangshen ES 1000W digital camera.