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Quorom q150r s sputter coater

Manufactured by Quorum Technologies
Sourced in United Kingdom

The Quorum Q150R S sputter coater is a piece of lab equipment designed for the deposition of thin films onto various substrates. It features a vacuum chamber and a sputtering process to apply these thin films.

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2 protocols using quorom q150r s sputter coater

1

Ependymal Lining Ultrastructure Visualization

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For Scanning Electron Microscopy the ependymal lining of the lateral ventricle was fixed in 2.5% glutaraldehyde, 2% paraformaldehyde in 0.1 m phosphate buffer, incubated in 2% osmium tetroxide, and dehydrated through ethanol solutions. Samples were critical point dried using an Emitech K850 (Quorum Technologies, East Sussex, UK), coated with platinum using a Quorom Q150R S sputter coater (Quorum Technologies). and visualised using a JEOL LSM-6010 scanning electron microscope (Jeol, Herts, UK).
Transmission electron microscopy was performed as previously described.22 (link)
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2

Scanning Electron Microscopy of Ependymal Lining

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For Scanning Electron Microscopy the ependymal lining of the lateral ventricle was fixed in 2.5% glutaraldehyde, 2% paraformaldehyde in 0.1 m phosphate buffer, incubated in 2% osmium tetroxide, and dehydrated through ethanol solutions. Samples were critical point dried using an Emitech K850 (Quorum Technologies, East Sussex, UK), coated with platinum using a Quorom Q150R S sputter coater (Quorum Technologies). and visualised using a JEOL LSM-6010 scanning electron microscope (Jeol, Herts, UK).
Transmission electron microscopy was performed as previously described.22 (link)
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