6890 gc system

Manufactured by Hewlett-Packard

Sourced in United States, Germany

The 6890 GC System is a gas chromatography instrument designed for the separation and analysis of chemical compounds. It features a temperature-controlled oven, a sample injection system, and a detector for identifying and quantifying the separated components. The core function of the 6890 GC System is to provide reliable and accurate separation and analysis of a wide range of samples across various industries.

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Lab products found in correlation

Cla methyl esters, by Merck Group (1 mentions) Flame ionization detector, by Agilent Technologies (1 mentions) Sp 2560 capillary column, by Merck Group (1 mentions)

Close spelling variants of this product

HP 6890 series GC system chromatograph HP 6890 GC Series System Plus HP 6890 Series GC System 6890 Series GC System GC 6890

3 protocols using 6890 gc system

Extraction and Quantification of PHB from Halomonas boliviensis

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Extraction and esterification of PHB from freeze-dried biomass of Halomonas boliviensis was carried out according to the method of Riis and Mai [27 ]. To 0.02 g of freeze dried biomass, 2 mL dichloroethane, 2 mL acidified propanol (propanol : HCl, 4 : 1, v/v), and 0.2 mL benzoic acid were added and the mixture was incubated for two hours at 100°C. After chilling to room temperature, 4 mL of demineralised water was added and the suspension was mixed. The organic phase was recovered after mixing.
Quantification of extracted PHB was performed by injection of 1 μL of the recovered organic phase on an Agilent PoraPLOT Q-HT (USA) column connected to a gas chromatography system (Hewlett Packard 6890 GC System, USA). Detection was carried out using a flame ionization detector with helium as carrier gas. Following temperature programme was used: initial temperature 90°C held for 2 minutes then increased to 150°C at a rate of 8°C minute⁻¹ and held for 4 minutes and finally increased to 280°C at 20°C minute⁻¹ and held for 1 minute. The retention time and peak area were compared to standards (PHB and benzoic acid) of known concentration.

Pleissner D., Lam W.C., Han W., Lau K.Y., Cheung L.C., Lee M.W., Lei H.M., Lo K.Y., Ng W.Y., Sun Z., Melikoglu M, & Lin C.S. (2014). Fermentative Polyhydroxybutyrate Production from a Novel Feedstock Derived from Bakery Waste. BioMed Research International, 2014, 819474.

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Gas Chromatography Analysis of Fatty Acids

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The composition of fatty acids was determined applying the method of gas chromatography with the help of Hewlett Packard 6890 GC System (Műnster, Germany) with a flame ionization detector (FID) in 100 m capillary column (produced by Chrompack, Middelburg, the Netherlands) with CP Sil 88 phase. The column diameter was 0.25 mm, and the film was 0.20 μm thick. The used separation conditions are presented in Table 2.
Identification of fatty acids was carried out based on the comparison of their retention time with the retention time of methyl esters of fatty acids of reference milk fat (BCR Reference Materials) of CRM 164 symbol. Examples of chromatograms are shown in Figure 1. The positional trans isomers of C18:1 were identified using the standards of methyl esters of these isomers (trans6, Supelco and trans9 and trans11, Sigma-Aldrich, St. Louis, MO, USA), whereas the trans isomers of C18:2 acid (cis,trans and trans,cis) were identified with the use of a mixture of standards of C18:2 isomers (Supelco). The cis9trans11 CLA isomer was identified using a mixture of CLA methyl esters (Sigma-Aldrich). The proportions of the individual acids were calculated by the ratio of their peak area to the total area of all identified acids (% mass fraction).

Paszczyk B., Polak-Śliwińska M, & Łuczyńska J. (2019). Fatty Acids Profile, Trans Isomers, and Lipid Quality Indices in Smoked and Unsmoked Cheeses and Cheese-Like Products. International Journal of Environmental Research and Public Health, 17(1), 71.

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Fatty Acid Methyl Ester Analysis Protocol

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The lipid fraction was extracted from 2 mL of MRS broth, skim milk, or gastrointestinal fluid using 20 mL of a 2:1 mixture of chloroform and methanol (Folch et al., 1957) . Subsequently, the lipids were reconstituted in 200 μL of chloroform, and 1 mL of 1 N methanolic hydrochloric acid was added. The mixture was allowed to react for 30 min at 60°C. The methylation reaction was stopped by addition of 200 μL of distilled water. The FAME were extracted twice in hexane (1 mL) and the extract was desiccated by addition of anhydrous sodium sulfate (Hernandez-Mendoza et al., 2009) . Then, FAME (1 μL) were injected into a Hewlett Packard 6890 GC system (Hewlett Packard, Wilmington, DE) equipped a SP-2560 capillary column (100 m × 0.25 mm, 0.2 μm thickness; Supelco, Bellefonte, PA) and a flame-ionization detector (Agilent Technologies, Wilmington, DE). The injector and detector temperatures were 240 and 260°C, respectively. Helium was used as the carrier gas with a constant flow of 1.5 mL/min with a split ratio of 1:5. The temperature program was as follows: 140°C for 4 min, to 176°C at 9°C/min, and to 180°C at 2°C/min. The total run time was 70 min. Methyl 9(Z),11(E)-octadecadienoate and methyl 10(E),12(Z)-octadecadienoate were used as analytical standards for the identification and determination of CLA. For CLA quantitation, a 5-point standard curve was constructed.

Sosa-Castañeda J., Hernández-Mendoza A., Astiazarán-García H., Garcia H.S., Estrada-Montoya M.C., González-Córdova A.F, & Vallejo-Cordoba B. (2015). Screening of Lactobacillus strains for their ability to produce conjugated linoleic acid in milk and to adhere to the intestinal tract. Journal of dairy science, 98(10).

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