Hematoxylin counterstain

Manufactured by Roche

Hematoxylin Counterstain is a laboratory reagent used in histology and cytology. It is a dye that stains nuclei in cells, providing contrast to the primary stain. The core function of Hematoxylin Counterstain is to enhance the visual distinction between cellular structures during microscopic examination.

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Lab products found in correlation

Dab map kit, by Roche (1 mentions) Bluing reagent, by Roche (1 mentions) Discovery xt system, by Roche (1 mentions) Bh 2 microscope, by Olympus (1 mentions) Anti 6x his tag, by Abcam (1 mentions)

Spelling variants of this product

Hematoxylin (52 citations) Hematoxylin Counterstain II (2 citations) Hematoxylin II counterstain reagent (2 citations)

2 protocols using hematoxylin counterstain

Immunohistochemical Staining of CYP27A1 in Liver

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The liver and heart tissues were stained with H&E. Immunohistochemical (IHC) staining for the CYP27A1 protein in liver tissue was conducted by using an autostain system with exclusive reagents (Discovery XT system; Ventana–Roche Diagnostics K.K., Basel, Switzerland). Deparaffinized 5-µm thick specimens of hepatic tissue were incubated with the primary antibody for anti-CYP27A1 (1:100) for 1 hour at room temperature, and then incubated with a biotin-conjugated universal secondary antibody (Ventana) for 32 min at 37 °C. It was developed using the DAB map kit (Ventana). The nucleus and cytoplasm were stained by using Hematoxylin Counterstain (Ventana) and a bluing reagent (Ventana). Specific immunoreaction of the primary antibody was confirmed by incubation without the primary antibody.

Miyazaki T., Sasaki S.I., Toyoda A., Wei F.Y., Shirai M., Morishita Y., Ikegami T., Tomizawa K, & Honda A. (2020). Impaired bile acid metabolism with defectives of mitochondrial-tRNA taurine modification and bile acid taurine conjugation in the taurine depleted cats. Scientific Reports, 10, 4915.

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Histological Analysis of SELP in Liver and Lungs

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Liver and lungs of each animal were immediately fixed in 10% buffered formalin for 48 hrs and then transferred to 70% ethanol. Sections from each liver lobe and each lung lobe were cut in 5 μm slices and analyzed. Tissue samples were stained using both hematoxylin and eosin (H&E) stain and an immunohistochemistry (IHC) stain specific for SELP using an antibody for poly-Histidine (anti-6X His tag^®, Abcam, Cambridge, MA; 1:3000 dilution), which is found in the carboxyl-terminal sequence of the SELP polymer strand. A chromogenic detection was used, IView diaminobenzidine research detection kit (Ventana Medical Systems) and hematoxylin counterstain (Ventana Medical Systems) in the IHC procedure. A piece of pre-gelled SELP was fixed, sliced, mounted onto a slide, and stained as a positive control using both stains. Slides were imaged using a Nikon DXM 1200C Digital Camera affixed to an Olympus BH2 microscope and analyzed in ACT-1C for DXM1200C software.

Poursaid A., Price R., Tiede A., Olson E., Huo E., McGill L., Ghandehari H, & Cappello J. (2015). In situ gelling silk-elastinlike protein polymer for transarterial chemoembolization. Biomaterials, 57, 142-152.

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