Mvx10 stereoscope

Manufactured by Olympus

Sourced in Japan

The MVX10 stereoscope is a binocular microscope designed for general laboratory applications. It provides a three-dimensional, magnified view of samples. The instrument features an optical system that allows for observation of specimens at various working distances.

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Lab products found in correlation

H1500, by Olympus (1 mentions) Qx100 ddpcr oil, by Bio-Rad (1 mentions) Embedding mold, by Polysciences (1 mentions) Jb 4 resin, by Merck Group (1 mentions) Focus pro, by Helicon (1 mentions)

4 protocols using mvx10 stereoscope

Oil-Based Microfluidic Chip for NASBA Reactions

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The SD chip was fabricated as described previously.^{27 (link)} An oil mixture composed of 0.04% Abil WE 09, 91% Tegosoft DEC, and 9% light mineral oil was prepared and pipetted into the inlet and outlet of the chip. A vacuum was used to load the oil onto the chip, displacing air from the channel and chamber array. The NASBA reaction mixture was added to the inlet, and flowed into the channel and chambers under vacuum. An additional oil mixture was then added to isolate the aqueous sample in compartmentalized volumes, digitizing the sample. For digitization tests, 2 μM calcein was added to the NASBA buffer to generate a fluorescent signal. Fluorescent photographs were acquired with an Olympus MVX10 stereoscope (Olympus Corporation, Shinjuku, Tokyo, Japan).

Wang J., Kreutz J.E., Thompson A.M., Qin Y., Sheen A.M., Wang J., Wu L., Xu S., Chang M., Raugi D.N., Smith R.A., Gottlieb G.S, & Chiu D.T. (2018). SD-Chip Enabled Quantitative Detection of HIV RNA using Digital Nucleic Acid Sequence-Based Amplification (dNASBA). Lab on a chip, 18(22), 3501-3506.

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Transcardial Perfusion and Brain Sectioning

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Anaesthetized mice were perfused transcardially with 4% PFA in PBS (pH 7.4). Brains were removed and further postfixed in 4% PFA in PBS at 4 °C overnight, after which the solution was replaced with PBS. They were kept at 4 °C until they were coronally sectioned (100-μm sections) with a Vibratome. Sections were mounted in Vectashield mounting medium containing DAPI (Vector Laboratories, H1500) and imaged with a camera (Olympus, DP72) attached to an MVX10 stereoscope (Olympus).

Miura S.K, & Scanziani M. (2022). Distinguishing externally from saccade-induced motion in visual cortex. Nature, 610(7930), 135-142.

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Droplet Digital PCR Emulsion Protocol

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For the experiments shown in Figure 2 (A–C), the oil mixture used was 68.3% mineral oil, 31.3% hexadecane, 0.4% Tegosoft DEC, and 0.018% Abil WE 09. The aqueous mixture was 1x Ssofast Evagreen Supermix with 2 mg/mL BSA, 500 nM generic primer, and fluorescein in 0.3× PBS. In Figure 2D the oil mixture was a 2:1 ratio of BioRad QX100 ddPCR oil and FC-40, and the aqueous mixture was the same as in Figure 2 (A–C). The device was primed with oil by placing oil in the inlet and outlet reservoirs, placing the entire device under vacuum to pull air out of the array, then removing the device from vacuum to allow oil to flow into the array. To digitize a sample, aqueous sample (~125% of the total array volume) was loaded into the inlet reservoir. Vacuum pressure (−0.3 bar) was applied until all of the aqueous sample had been drawn through the device, followed by oil which displaced aqueous sample in the channels (2–10 minutes). Videos and bright field and fluorescence photographs were obtained on an Olympus MVX10 stereoscope with an Olympus MV PLAPO 0.63x objective.

Kreutz J.E., Wang J., Sheen A.M., Thompson A.M., Staheli J.P., Dyen M.R., Feng Q, & Chiu D.T. (2019). Self-Digitization Chip for Quantitative Detection of Human Papillomavirus Gene Using Digital LAMP. Lab on a chip, 19(6), 1035-1040.

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Mineralized Tissue Visualization and Histological Analysis

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Visualization of mineralized tissues was carried out as described previously (63 ). All photographs were taken in numerous focal planes with Olympus MVX10 stereoscope with AxioCam and the final images were prepared with Helicon Focus Pro (HeliconSoft), allowing to form high-resolution images. Mineralized tissues for histological analysis were washed in distilled water and placed in Morse’s solution (10% sodium citrate and 22.5% formic acid) at room temperature for decalcification until the tissues were soft. Next, the samples were dehydrated in 100% ethanol and incubated in an infiltration solution of JB-4 resin (prepared according to the manufacturer’s instructions; Sigma-Aldrich) at room temperature overnight. The next day, the infiltration solution was replaced by an embedding solution (prepared according to the manufacturer’s instructions), placed into an embedding mold (PolyScience), and transferred to a vacuum chamber which accelerated the polymerization (~3 h). The resin block was sectioned at 5 μm, and sections were stained with Mayer’s hematoxylin (except SI Appendix, Fig. S2I) or Verde Luz-orange G-acid fuchsin (VOF) stain (SI Appendix, Fig. S2I).

Stundl J., Martik M.L., Chen D., Raja D.A., Franěk R., Pospisilova A., Pšenička M., Metscher B.D., Braasch I., Haitina T., Cerny R., Ahlberg P.E, & Bronner M.E. (2023). Ancient vertebrate dermal armor evolved from trunk neural crest. Proceedings of the National Academy of Sciences of the United States of America, 120(30), e2221120120.

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