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Paragyline

Manufactured by Cayman Chemical
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Paragyline is a selective, irreversible inhibitor of monoamine oxidase type A (MAO-A) enzyme. It is used as a research tool to study the role of MAO-A in various biological processes.

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Lab products found in correlation

5 azacytidine, by Cayman Chemical (2 mentions) Tmp269, by Selleck Chemicals (2 mentions) Anacardic acid, by Cayman Chemical (2 mentions) Ms 275, by Merck Group (2 mentions) Rgfp966, by Selleck Chemicals (2 mentions) Pci 34051, by Selleck Chemicals (2 mentions)

2 protocols using paragyline

1

HDAC Inhibition and TNF-α Secretion Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
For the HDAC inhibition assay, cells were pretreated with TSA (5nM, Sigma-Aldrich). For the TNF-α secretion assay, human THP1 or murine macrophage RAW264.7 cells were treated with TSA, VPA (0.1 μM), Ms275 (5 μM), NIC (0.5 μM) HPOB (0.5 μM) (Sigma-Aldrich), TMP269 (0.1 μM), PCI-34051 (0.1 μM), RGFP966 (0.5 μM) (Selleckchem), paragyline (3 μM), 5'azacytidine (10 μM) or anacardic acid (10 μM) (Cayman Chemical). The concentration of HDAC inhibitors used did not induce cytotoxicity (Supplementary Fig.5 a-h). It is important to note that at high concentrations, HDAC inhibitors blocked TNF-α secretion (Supplementary Fig. 7b-i) 30 (link).
Bandyopadhaya A., Tsurumi A., Maura D., Jeffrey K.L, & Rahme L.G. (2016). A Quorum Sensing Signal Promotes Host Tolerance Training Through HDAC1-Mediated Epigenetic Reprogramming. Nature microbiology, 1, 16174.
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2

HDAC Inhibition and TNF-α Secretion Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
For the HDAC inhibition assay, cells were pretreated with TSA (5nM, Sigma-Aldrich). For the TNF-α secretion assay, human THP1 or murine macrophage RAW264.7 cells were treated with TSA, VPA (0.1 μM), Ms275 (5 μM), NIC (0.5 μM) HPOB (0.5 μM) (Sigma-Aldrich), TMP269 (0.1 μM), PCI-34051 (0.1 μM), RGFP966 (0.5 μM) (Selleckchem), paragyline (3 μM), 5'azacytidine (10 μM) or anacardic acid (10 μM) (Cayman Chemical). The concentration of HDAC inhibitors used did not induce cytotoxicity (Supplementary Fig.5 a-h). It is important to note that at high concentrations, HDAC inhibitors blocked TNF-α secretion (Supplementary Fig. 7b-i) 30 (link).
Bandyopadhaya A., Tsurumi A., Maura D., Jeffrey K.L, & Rahme L.G. (2016). A Quorum Sensing Signal Promotes Host Tolerance Training Through HDAC1-Mediated Epigenetic Reprogramming. Nature microbiology, 1, 16174.
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