LINC01089 sequence (NR_002809.3) was synthesized by Genewiz (Suzhou, China) and inserted into pcDNA3.1 (ov-LINC01089). Empty pcDNA3.1 vector served as a negative control (ov-NC). Three LINC01089 siRNAs (si-LINC01089), negative control (si-NC), miR-3187-3p mimic and NC mimic were synthesized by Genepharma (Shanghai, China). ov- LINC01089, ov-NC, miR-3187-3p mimic, and NC mimic were transfected into cells using Lipofectamine 3000 (Life Technologies, Gaithersburg, MD, USA).
Si linc01089
Manufactured by GenePharma
Sourced in China
Si-LINC01089 is a laboratory equipment product. It is designed to facilitate the study and analysis of the LINC01089 gene. The core function of Si-LINC01089 is to enable the silencing or downregulation of the LINC01089 gene expression in cell-based experiments.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Pcdna empty vector, by GenePharma (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Mir 27b 3p mimic, by GenePharma (1 mentions)
Pcdna empty vector, by Vector Laboratories (1 mentions)
Pcdna linc01089, by Vector Laboratories (1 mentions)
3 protocols using si linc01089
1
Overexpression and Silencing of LINC01089
2
LINC01089 Overexpression in HCC Cells
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Human HCC cell lines, Huh-7 and LM3, were purchased from the Cell Bank of Chinese Academy of Sciences (Shanghai, China). Cell lines were cultured in DMEM supplemented with 10% fetal bovine serum (FBS) and kept in a humidified atmosphere at 37 °C with 5% CO2.
The pcDNA empty vector (Vector), pcDNA-LINC01089 overexpression vector (LINC01089), siRNA negative control (si-NC), and siRNAs against LINC01089 (si-LINC01089) were provided by GenePharma (Shanghai, China). Cell transfection was carried out according to the manufacturer’s instructions. After 6 h post-transfection, cells were cultured in fresh complete medium for subsequent experiments.
The pcDNA empty vector (Vector), pcDNA-LINC01089 overexpression vector (LINC01089), siRNA negative control (si-NC), and siRNAs against LINC01089 (si-LINC01089) were provided by GenePharma (Shanghai, China). Cell transfection was carried out according to the manufacturer’s instructions. After 6 h post-transfection, cells were cultured in fresh complete medium for subsequent experiments.
3
LINC01089 Functional Regulation in CRC
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pcDNA empty vector (Vector), pcDNA-LINC01089 (LINC01089), siRNA normal control (si-NC), siRNAs against LINC01089 (si-LINC01089), miR-27b-3p mimic, and miR-27b-3p inhibitors were provided by GenePharma Co., Ltd. (Shanghai, China). SW480, HT29, SW620 and LoVo cells were harvested, re-suspended with serum-free medium and inoculated into a 6-well cell culture plate (2×105/well). After 24h of culture, the CRC cells were transfected with Lipofectamine ® 2000 (Invitrogen, Carlsbad, CA, USA) according to the supplier’s instructions. 12 h later, complete medium was added to continue the culture. After 24h, quantitative real-time polymerase chain reaction (qRT-PCR) was utilized to investigate the transfection efficiency.
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