Multi species tgf β elisa kit

Peripheral blood samples (3-4 mL) from 17 cirrhotic patients were drawn before and after treatment with PPIs. Blood samples were centrifuged and the sera were collected and stored aliquoted at -80°C until further use. The serum levels of cytokines IL-1β, -6, -8, -10, -12p70 and TNF-α were determined by a cytometric bead array method, using the Human Inflammatory Cytokine CBA kit (BD Bioscience, San Diego, USA). The measurements were performed on a BD FACS Array Bioanalyzer. The raw data were analyzed using the Flow Jo V7.5 software (Tree Star Inc., Ashland, OR, USA). Further analysis of the data was performed using the Curve Expert 1.4 software. Serum levels of TGF-β and LBP were determined by ELISA, using a multi-species TGF-β ELISA kit (Invitrogen Corporation, CA, USA) and a human LBP ELISA kit (SunRed Biological Technology, Shanghai). NOx levels were measured by a nitric oxide quantitation kit (Active Motif, Belgium). Endotoxin levels were measured using a LAL Chromogenic Endpoint assay (Hycult biotech, The Netherlands). Data analysis was performed using the Curve Expert 1.4 software. The detection limits of the methods employed were: IL-8=3.6 pg/mL, IL-1β=7.2 pg/mL, IL-6=2.5 pg/mL, IL-10=3.3 pg/mL, TNF-α=3.7 pg/mL, IL-12p70=1.9 pg/mL, LBP=0.135 μg/mL, TGF-β=15.6 pg/mL, NOx <1 μM, endotoxin=0.04 EU/mL.