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Ca2.1d6

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The CA2.1D6 is a laboratory equipment product manufactured by Bio-Rad. It serves as a core function in various laboratory applications. No further details or interpretation on its intended use can be provided in an unbiased and factual manner.

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Lab products found in correlation

Fjk 16, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 goat anti rat, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 goat anti mouse igg2a, by Thermo Fisher Scientific (1 mentions) Alexa fluor 647 goat anti mouse igg1, by Thermo Fisher Scientific (1 mentions) Clone 3 4f4, by Southern Biotech (1 mentions) Facsaria sorp instrument, by BD (1 mentions)

3 protocols using ca2.1d6

1

Feline Immune Cell Phenotyping

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Monoclonal antibodies against the epsilon chain of feline CD3 (NZM1) and against feline CD56 (SZK1) were kindly provided by Dr. Yorihiro Nishimura (Tokyo University, Japan)45 (link). Monoclonal antibodies FE5.4D2, and CA2.1D6 recognising feline CD8β, and canine CD21, respectively, were purchased from Bio-Rad. A monoclonal antibody (FJK-16s), directly conjugated with Alexa fluor 647 (AF647) and cross-reacting with feline Foxp3 was purchased from eBioscience. Monoclonal antibody CAT30A against feline CD4 was purchased from Veterinary Medical Research and Development (VMRD). Conjugated secondary antibodies (Invitrogen) were goat anti-rat Alexa Fluor 488, goat anti-mouse IgG R-Phycoerythrin, goat anti-mouse IgG2a Alexa Fluor 488, goat anti-mouse IgG1 Alexa Fluor 647 and goat anti-mouse IgG3 fluorescein isothiocyanate (FITC). When primary antibodies from the same IgG1 isotype were used, one primary antibody was labeled with Zenon Alexa Fluor 488 Mouse IgG1.
Desmarets L.M., Vermeulen B.L., Theuns S., Conceição-Neto N., Zeller M., Roukaerts I.D., Acar D.D., Olyslaegers D.A., Van Ranst M., Matthijnssens J, & Nauwynck H.J. (2016). Experimental feline enteric coronavirus infection reveals an aberrant infection pattern and shedding of mutants with impaired infectivity in enterocyte cultures. Scientific Reports, 6, 20022.
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2

Feline Lymphocyte Immunophenotyping

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Whole blood from each cat was collected into EDTA anti-coagulant tubes at various times post infection for a complete blood count with differential and lymphocyte phenotype analysis. Percentages of CD4 + and CD8 + T lymphocytes were determined by two- and three-color flow cytometry. The fluorochrome-conjugated monoclonal antibodies used in staining were PE-conjugated anti-CD3(mAb 572, NCSU Hybridoma), FITC-conjugated anti-CD4 (mAb 30A, NCSU Hybridoma), PE-conjugated anti-CD8 (mAb 3.357, NCSU Hybridoma), and PE-conjugated anti-CD21 mAb (Biorad, CA2.1D6)). Isotype-matched irrelevant antibodies were used as controls. The percent of positively stained lymphocytes was determined using a Becton Dickinson LSR II flow cytometry unit. Approximately 20,000 gated events were acquired and analyzed using FlowJo software. Complete blood cell counts were determined by the veterinary clinical diagnostic laboratory at NCSU-CVM and the absolute lymphocyte number determined by differential counts. The number of cells within each lymphocyte subpopulation was then calculated based on the subset percentages obtained by flow cytometry and the absolute lymphocyte count reported on the CBC.
Fogle J.E., Hudson L., Thomson A., Sherman B., Gruen M., Lacelles B.D., Colby B.M., Clary G., Longo F, & Meeker R.B. (2021). Improved neurocognitive performance in FIV infected cats following treatment with the p75 neurotrophin receptor ligand LM11A-31. Journal of neurovirology, 27(2), 302-324.
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3

Circulating Lymphocyte Phenotyping in Felines

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Flow cytometry analysis of circulating lymphocyte lineages was performed as previously described [41 (link)]. Briefly, 50 μL of EDTA-treated blood was treated with mouse monoclonal antibodies targeting CD4, CD8 and CD21 feline cell markers (Fisher, clone 3-4F4, FITC; Southern Biotech, clone fCD8, PE; and Bio-Rad, CA2.1D6, AF647 respectively) to determine the percentage of each cell type. Unstained and single stained samples were used as controls. BD FACSAria™ SORP instrument (Becton Dickinson, San Jose, CA, USA) containing BD FACSDiva™ Software (Diva 9.0.1., San Jose, CA, USA) was used to obtain data, and were analyzed using FlowJo 10.8.0. (Ashland, OR, USA).
Weerarathne P., Maker R., Huang C., Taylor B., Cowan S.R., Hyatt J., Tamil Selvan M., Shatnawi S., Thomas J.E., Meinkoth J.H., Scimeca R., Birkenheuer A., Liu L., Reichard M.V, & Miller C.A. (2023). A Novel Vaccine Strategy to Prevent Cytauxzoonosis in Domestic Cats. Vaccines, 11(3), 573.
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