R848, MyD88 knockdown constructs, and noble agar were from Sigma (St Louis, MO, USA). SB203580, PD08959, and SP600125 were obtained from Selleck Chem (Boston MA, USA). MTT reagent was obtained in the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega Madison WI, USA). p-p38, MyD88, α-tubulin, and actin antibodies were obtained from Cell Signaling Technologies (Beverly MA, USA). GAPDH was from Santa Cruz (Carlsbad, CA, USA). CD11b and CD14 antibodies were obtained from EBiosciences (San Diego CA, USA). Anti-Il-8 antibody was obtained from Abcam (Cambridge MA, USA). OCI-AML3 was obtained from DSMZ (Braunschweig, Germany) and 293T, HCT116, HL60, THP-1, and U937 cells were obtained from ATCC (Manassas, VA, USA). Primary AML human bone marrow cells were obtained from the CWRU Hematopoietic Stem Cell Core Facility (Cleveland, OH, USA). The cDNA for TLR8 was cloned into the pLVX-EF1alpha-IRES-mCherry vector obtained from Clontech (Mountain View, CA, USA)
Pd08959
Manufactured by Selleck Chemicals
Sourced in United States
PD08959 is a laboratory instrument designed for conducting various scientific experiments and analyses. It is a multi-purpose device that can be used for a range of applications within the research and development field. The core function of this product is to provide precise and reliable data collection and analysis capabilities to support the needs of scientific professionals.
Automatically generated - may contain errors
Lab products found in correlation
Noble agar, by Merck Group (2 mentions)
Myd88, by Cell Signaling Technology (2 mentions)
Mtt reagent, by Promega (2 mentions)
Anti il 8 antibody, by Abcam (2 mentions)
Thp 1, by American Type Culture Collection (2 mentions)
Hl 60, by American Type Culture Collection (2 mentions)
α tubulin, by Cell Signaling Technology (2 mentions)
Myd88 knockdown constructs, by Merck Group (2 mentions)
Cd11b and cd14 antibodies, by Thermo Fisher Scientific (2 mentions)
Plvx ef1alpha ires mcherry vector, by Takara Bio (2 mentions)
Hct116, by American Type Culture Collection (2 mentions)
Sp600125, by Selleck Chemicals (2 mentions)
Sb203580, by Selleck Chemicals (2 mentions)
Oci aml3, by Leibniz Institute DSMZ (2 mentions)
Gapdh, by Santa Cruz Biotechnology (2 mentions)
Celltiter 96 aqueous one solution cell proliferation assay, by Promega (2 mentions)
2 protocols using pd08959
1
Investigating TLR8 Signaling in AML
2
Investigating TLR8 Signaling in AML
Check if the same lab product or an alternative is used in the 5 most similar protocols
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R848, MyD88 knockdown constructs, and noble agar were from Sigma (St Louis, MO, USA). SB203580, PD08959, and SP600125 were obtained from Selleck Chem (Boston MA, USA). MTT reagent was obtained in the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega Madison WI, USA). p-p38, MyD88, α-tubulin, and actin antibodies were obtained from Cell Signaling Technologies (Beverly MA, USA). GAPDH was from Santa Cruz (Carlsbad, CA, USA). CD11b and CD14 antibodies were obtained from EBiosciences (San Diego CA, USA). Anti-Il-8 antibody was obtained from Abcam (Cambridge MA, USA). OCI-AML3 was obtained from DSMZ (Braunschweig, Germany) and 293T, HCT116, HL60, THP-1, and U937 cells were obtained from ATCC (Manassas, VA, USA). Primary AML human bone marrow cells were obtained from the CWRU Hematopoietic Stem Cell Core Facility (Cleveland, OH, USA). The cDNA for TLR8 was cloned into the pLVX-EF1alpha-IRES-mCherry vector obtained from Clontech (Mountain View, CA, USA)
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