Nunc maxisorp flat bottom 96 well elisa plates

Manufactured by Thermo Fisher Scientific

The Nunc MaxiSorp® flat-bottom 96 well ELISA plates are a type of laboratory equipment used for enzyme-linked immunosorbent assay (ELISA) applications. The plates feature a flat-bottom design and a high-affinity MaxiSorp surface, which is optimized for protein binding.

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Lab products found in correlation

Attophos ap fluorescent substrate system, by Promega (2 mentions) Fluostar omega fluorescent plate reader, by BMG Labtech (2 mentions) Recombinant fgf19, by R&D Systems (1 mentions) Streptavidin hrp conjugate, by Thermo Fisher Scientific (1 mentions) Synergy h1, by Agilent Technologies (1 mentions) Tmb substrate, by Merck Group (1 mentions)

Close spelling variants of this product

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3 protocols using nunc maxisorp flat bottom 96 well elisa plates

FGFR4-FGF19 Binding Inhibition Assay

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Example 12

Nunc MaxiSorp® flat-bottom 96 well ELISA plates purchased from Thermo Fisher Scientific were coated with recombinant human FGFR4 (extracellular domain, Fc chimera; acquired from R&D Systems at a final concentration of 4 μg/well and processed according to manufacturer's instructions. Each well was incubated with 1.2 μg/ml of recombinant FGF-19 from R&D Systems, followed by treatment with indicated concentration of anti-FGFR4 antibody U4-3. After incubation and washes, bound FGF-19 was detected with 0.2 μg/ml of biotinylated anti-FGF19 antibody (R&D Systems) followed by incubation with alkaline-phosphatase conjugated Streptavidin. Enzymatic reaction was triggered by incubation with the AttoPhos® AP Fluorescent Substrate System (Promega and detected with the Fluostar Omega fluorescent plate reader from BMG Labtech. The data show that U4-3 is able to effectively compete the binding of FGF-19 to the receptor. The results are shown in FIG. 9.

US10358498B2. Human anti-FGFR4 antibody (2019-07-23). Daiichi Sankyo Europe GmbH [DE], Daiichi Sankyo Company, Limited [JP]. Inventors: Reimar Abraham [DE], Keisuke Fukuchi [JP], Tanja Lange [DE], Johannes Bange [DE], Ichiro Watanabe [JP], Shinko Hayashi [JP], Toshiaki Ohtsuka [JP].

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FGFR4 Antibody Binding Assay

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Example 8

Nunc MaxiSorp® flat-bottom 96 well ELISA plates purchased from Thermo Fisher Scientific were coated with recombinant human FGFR (or BSA as control) as indicated (extracellular domain, Fc chimera) acquired from R&D Systems at a final concentration of 100 μg/well and processed according to manufacturer's instructions. After incubation with anti-FGFR4 antibody U4-3 as indicated, binding was detected with an alkaline-phosphatase coupled goat anti-human IgG F(ab′) using the AttoPhos® AP Fluorescent Substrate System purchased from Promega and detected with the Fluostar Omega fluorescent plate reader from BMG Labtech. The results are shown in FIG. 5.

The antibody U4-3 recognizes the human FGFR4 with high specificity and—importantly does not cross-react with other members of the FGFR family.

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Binding Analysis of AMA1-RON2L Immunogens

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Binding of the AMA1 DI-DII and single-component immunogens to the IgNAR 14I-1 and RON2L were analyzed by ELISA. Immunogens were diluted in 50 mM Na-carbonate (pH 9.5) and coated on Nunc MaxiSorp flat-bottom 96-well ELISA plates (Thermo Fisher Scientific, Cat# 44-2404-21) at 10 nM in 100 μl at 4 °C overnight. The plates were then washed three times with PBS containing 0.05% Tween 20 (PBS/T), blocked with 2% bovine serum albumin in PBS/T for 1 h at room temperature, and then washed three times with PBS/T. Next, 200 μl of biotinylated 14I-1 or TrxA-RON2L-2 diluted to 200 nM and 1000 nM in blocking buffer (PBS/T with 2% bovine serum albumin) was added to each well of the blocked plates, incubated for 1 h at room temperature, and then washed three times with PBS/T. Then, 200 μl of streptavidin HRP conjugate (Thermo Fisher Scientific, Cat# 21127) was added to each well at a 1:10000 dilution and incubated for 1 h at room temperature. The plates were then washed three times with PBS/T and developed with 70 μl of TMB substrate (MilliporeSigma) for 20 min at room temperature in the dark. The reaction was then stopped by adding 160 mM sulfuric acid (H₂SO₄), and the absorbance was measured at 450 nm on a BioTek™ Synergy H1 microplate reader using Gen5 3.08.01 software.

Patel P.N., Dickey T.H., Diouf A., Salinas N.D., McAleese H., Ouahes T., Long C.A., Miura K., Lambert L.E, & Tolia N.H. (2023). Structure-based design of a strain transcending AMA1-RON2L malaria vaccine. Nature Communications, 14, 5345.

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