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3 protocols using 3 5 dicaffeoyl quinic

1

Quantitative Analysis of Plant Phenolic Acids

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Methanol HPLC LC-MS Grade (CAS no. 67-56-1) and acetic acid (CAS no. 64-19-7) of HPLC Grade were purchased in Merck (Darmstadt, Germany). Methanol (CAS no. 67-56-1) for plant material extraction purchased in Avantor Performance Materials (Poland). Standard substances of rosmarinic (CAS no. 20283-92-5), chlorogenic (CAS no. 327-97-9), caffeic (CAS no. 331-39-5), ferulic (CAS no. 1135-24-6), 3,5-dicaffeoyl-quinic (CAS no. 2450-53-5), sinapic (CAS no. 530-59-6) and p-coumaric (CAS no. 501-98-4) acids were purchased in Sigma-Aldrich (Poznań, Poland); 5-O-feruoylouinic acid (CAS no. 1135-24-6) was purchased in LCG Standards Poland. Lithospermic B acid (CAS 115939-25-8), shikonin (CAS no. 517-88-4), acetylshikonin (CAS no. 24502-78-1), isobutyrylshikonin (CAS no. 52438-12-7), deoxyshikonin (CAS no. 43043-74-9) and isovalerylshikonin (CAS no. 52387-14-1) were purchased in ChemFaces (Wuhan, China).
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2

Analytical Standards for Phenolic Compounds

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The following solvents were used in the present study: 99.9% acetonitrile, 99.9% methanol, obtained from Sigma-Aldrich (Steinheim, Germany) and 99.8% trifluoroacetic acid supplied from Merck (Darmstadt, Germany). The water was purified using a Millipore Milli-Q apparatus. Analytical and HPLC grade substances: potassium persulphate, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), caffeic, neochlorogenic, chlorogenic, 4-caffeoylquinic, 3,4-dicaffeoylquinic, 3,5-dicaffeoylquinic, 1,5-dicaffeoylquinic, and 4,5-dicaffeoylquinic acids, rutin, quercitrin, quercetin, isoquercitrin, luteolin-7-glucoside, luteolin-7-rutinoside, luteolin-3,7-diglucoside, apigenin-7-glucoside, and santin were obtained from Sigma-Aldrich; luteolin from Roth GmbH (Karlsruhe, Germany); trolox and apigenin from Fluka Chemika (Buchs, Switzerland).
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3

HPLC Analysis of Phenolic Compounds

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Methanol (CAS no. 67-56-1), acetonitrile (CAS no. 75-05-8), and acetic acid (CAS no. 64-19-7) of HPLC Grade were purchased in Merck (Germany). Deionized water was obtained from Simplicity Millipore water purification system (Merck, Germany). While standard substances of chlorogenic (CAS no. 327-97-9), caffeic (CAS no. 331-39-5), ferulic (CAS no. 1135-24-6), 3,5-dicaffeoyl-quinic (CAS 2450-53-5), oleanolic acid (CAS 508-02-1), sinapic (CAS 530-59-6), and p-coumaric (CAS) acids were purchased in Sigma-Aldrich (Poznań, Poland); 5-O-feruoylouinic acid (CAS) 1135-24-6) was purchased in LCG Standards (Lomianki, Poland). Methanol (CAS no. 67-56-1) for plant material extraction was purchased from Avantor Performance Materials (Gliwice, Poland).
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