Antibodies used in the study included the following: rabbit anti-YTHDC1 (GTX32976, GeneTex, USA); rabbit anti-YTHDF2 (24744-1-AP, proteintech); rabbit anti-METTL3 (15073-1-AP, proteintech); mouse anti-Flag-tag (F1804, SigmaAldrich, Saint Louis, MO, USA); mouse anti-HA-tag (M180-3, MBL, Japan); mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (CB100127, California Bioscience, Coachella, CA, USA); rabbit anti-IAV NP, NS1, and NEP, (GTX125989, GTX125990, and GTX125953, GeneTex, USA); mouse anti-NP (produced in our laboratory); control rabbit IgG polyclonal (AC005, ABclonal Biotechnology, Cambridge, MA, USA); horseradish peroxidase-conjugated anti-mouse and anti-rabbit (BF03001 and BF03008, Beijing Biodragon Inmmunotechnologies, China); Alexa Fluor 594-conjugated goat anti-rabbit (GR200G-43C, Sungene Biotech); fluorescein isothiocyanate (FITC) goat anti-mouse (GM200G-02C, Sungene Biotech); and FITC-goat anti-rabbit (GR200G-02C, Sungene Biotech). 4′,6′-diamidino-2-phenylindole (1:1000) (no. C1002) was purchased from the Beyotime, China.
Gtx32976
Manufactured by GeneTex
Sourced in United States
The GTX32976 is a laboratory equipment product manufactured by GeneTex. It is designed to perform a specific core function within a research or testing environment. The details of its intended use and capabilities are not available in this factual and unbiased description.
Automatically generated - may contain errors
Lab products found in correlation
Gtx125989, by GeneTex (1 mentions)
Gtx125953, by GeneTex (1 mentions)
Gr200g 43c, by Sungene Biotech (1 mentions)
Gm200g 02c, by Sungene Biotech (1 mentions)
Gr200g 02c, by Sungene Biotech (1 mentions)
Gtx125990, by GeneTex (1 mentions)
Mouse anti flag tag, by Merck Group (1 mentions)
Bradford reagent, by Bio-Rad (1 mentions)
Proteinase and phosphatase inhibitor, by Roche (1 mentions)
Hpa038002, by Merck Group (1 mentions)
Hpa007196, by Merck Group (1 mentions)
Ab103328, by Abcam (1 mentions)
Ab92821, by Abcam (1 mentions)
Hrp conjugated secondary antibody, by Abcam (1 mentions)
Hpa037364, by Merck Group (1 mentions)
2 protocols using gtx32976
1
Comprehensive Antibody Panel for Molecular Analyses
2
Quantitative Analysis of m6A Regulators
Check if the same lab product or an alternative is used in the 5 most similar protocols
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NPC cell lines were lysed in a standard RIPA buffer containing proteinase and phosphatase inhibitors (Roche, Indianapolis, IN, USA), and the protein concentrations were quantified using Bradford reagent (Bio-Rad). Then prepared protein was separated via SDS-PAGE and transferred to NC membrane (Pierce Biotechnologies Inc., Rockford, IL, USA). The primary antibodies used in the study include anti-METTL3 (15073-1-AP, Proteintech), METTL14 (HPA038002, Sigma), FTO(ab92821, Abcam), ALKBH5 (HPA007196, Sigma), WTAP (sc-374280, Santa Curz), YTHDF1 (17479-1-AP, Proteintech), YTHDF2 (24744-1-AP, Proteintech), YTHDF3 (ab103328, Abcam), YTHDC1 (GTX32976, GeneTex), YTHDC2 (HPA037364, Sigma), IGF1R (3027,CST), AKT (9272, CST), Phospho-AKT (ser473, CST), S6 Ribosomal protein (2217, CST), and Phospho-S6 Ribosomal protein (ser240/244)(2215, CST). The bound antibodies were detected with HRP-conjugated secondary antibodies (Abcam). Densitometric analysis of WB results was performed with ImageJ software.
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