Temcam f416 4 k 4 k ccd camera

A Zeiss Gemini 1530 (Zeiss Oberkochem; Germany) was applied for capturing high-resolution SEM (scanning electron microscope) images. For the lower magnifications, an ESEM (environmental scanning electron microscope) with an ESEM XL-30 microscope (Philips, Eindhoven; Netherlands) was used. Samples for electron microscopic SEM/ESEM analyses were fixed in 2% [v/v] aqueous glutaraldehyde fixative and finally treated with osmium oxide. Then, the specimens were processed through acetone dehydration steps and subjected to critical point drying at 43 °C. The TEM (transmission electron microscope) analyses were performed with a TemCam-F416 (4 K × 4 K) CCD camera (TVIPS, Gauting; Germany) hooked to a Tecnai 12 transmission electron microscope (FEI, Eindhoven; The Netherlands), by using an accelerating voltage of 120 kV.
A digital light microscope VHX-600 (Keyence, Neu-Isenburg; Germany) with a VH-Z25 zoom lens was applied for light microscopic inspections.

Electron micrographs were taken by a TemCam-F416 4K 4K CCD camera (TVIPS, Gauting, Germany) operated on a FEI Tecnai12 transmission electron microscope at 120 kV. In order to view the cells in 3D [53] (link), [54] (link), tilt series over a tilt range of −60° to +60° with 1.5° increments were recorded. The magnification of 23,000× (0.929 nm pixel size on the CCD camera) was binned by the factor 2 (2048×2048 pixels micrographs). The EM-MENU 4 software package (TVIPS, Gauting, Germany) was used to collect the tilt series.