Wga fitc conjugate

Manufactured by Merck Group

Sourced in France

The WGA-FITC conjugate is a fluorescently labeled lectin product used in various research applications. It consists of wheat germ agglutinin (WGA) covalently linked to the fluorescent dye fluorescein isothiocyanate (FITC). The conjugate binds to N-acetylglucosamine and sialic acid residues on cell surfaces, enabling visualization and detection of these moieties in biological samples.

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Lab products found in correlation

N acetyl d galactosamine galnac, by Merck Group (1 mentions) Porcine mucin, by Merck Group (1 mentions) Calcofluor white m2r, by Merck Group (1 mentions) N acetyl d glucosamine glcnac, by Merck Group (1 mentions) Mouse anti flotillin 1, by BD (1 mentions) Ab2219, by Merck Group (1 mentions) A0021, by Agilent Technologies (1 mentions) A 0061, by Agilent Technologies (1 mentions) Mouse anti eea1, by BD (1 mentions) Rabbit anti rfp, by Rockland Immunochemicals (1 mentions) Ab84903, by Abcam (1 mentions) Mouse anti β actin, by Merck Group (1 mentions) Mouse anti α tubulin, by Merck Group (1 mentions) Rabbit anti gapdh, by Cell Signaling Technology (1 mentions) Ab40873, by Abcam (1 mentions) Rabbit anti egfr, by Santa Cruz Biotechnology (1 mentions) C2 plus, by Nikon (1 mentions)

Spelling variants of this product

WGA-FITC (50 citations) FITC conjugate (7 citations)

3 protocols using wga fitc conjugate

Glycan Analysis of Porcine Mucin

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Calcofluor white M2R (Calcofluor; F3543), N-Acetyl-D-glucosamine (GlcNAc; A4106), N-Acetyl-D-galactosamine (GalNAc; A2795), D-(+)-Fucose (Fucose; F8150), D-(+)-Mannose (Mannose; M6020), Mucin from porcine stomach (Porcine mucin; M2378) and WGA-FITC conjugate (L4895) were all purchased from Sigma-Aldrich (Lyon, France).

Pigeyre L., Schatz M., Ravallec M., Gasmi L., Nègre N., Clouet C., Seveno M., El Koulali K., Decourcelle M., Guerardel Y., Cot D., Dupressoir T., Gosselin-Grenet A.S, & Ogliastro M. (2019). Interaction of a Densovirus with Glycans of the Peritrophic Matrix Mediates Oral Infection of the Lepidopteran Pest Spodoptera frugiperda. Viruses, 11(9), 870.

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Antibody Immunodetection Protocol

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The following antibodies were used: rabbit anti-human transferrin (A0061, Dako); rabbit anti-human Gc-globulin (also known as VDBP, A0021, Dako); rabbit anti-uteroglobin (also known as CC16, ab40873, Abcam); rabbit anti-SLC1A5 (also known as SGLT2, ab84903, Abcam); rabbit NaPi-IIa (gift from C.A.Wagner, University of Zurich, Zurich, Switzerland); rabbit anti-human AQP1 (ab2219, Millipore); mouse anti-β-actin (A2228, Sigma-Aldrich); mouse conjugated to Fluorescein (FITC) anti-PI(4,5)P₂ (Z-G045, Echelon Biosciences Inc.); mouse anti-EEA1 (610456, BD Bioscience); rabbit anti-RFP (600–401-379, ROCKLAND); sheep anti-LRP2 (gift from P. Verroust and R. Kozyraki, INSERM, Paris, France); mouse anti Flotillin-1(610821, BD Bioscience); mouse anti-α-tubulin (T5168, Sigma-Aldrich); rabbit anti-GAPDH (2118, Cell Signaling Technology); rat anti-LAMP1 (sc-19992, Santa Cruz Biotechnology); goat anti-Cathepsin-D (Cts-D; sc-6486, Santa Cruz Biotechnology); rabbit anti-EGFR (1005 sc-03, Santa Cruz Biotechnology); Alexa-488 Phalloidin (F-actin, A12379, Thermofisher Scientific); mouse anti-Transferrin Receptor Antibody (H68,4, ThermoFisher Scientific); WGA FITC Conjugate (L 4895, Sigma-Aldrich); mouse anti-Na⁺/K⁺-ATPase subunit α1 (C464.6 EMD Millipore); rabbit anti-MPR and rabbit anti-OCRL (gift from A. De Matteis, Telethon Institute of Genetics and Medicine (TIGEM), Pozzuoli, Italy).

Festa B.P., Berquez M., Gassama A., Amrein I., Ismail H.M., Samardzija M., Staiano L., Luciani A., Grimm C., Nussbaum R.L., De Matteis M.A., Dorchies O.M., Scapozza L., Wolfer D.P, & Devuyst O. (2018). OCRL deficiency impairs endolysosomal function in a humanized mouse model for Lowe syndrome and Dent disease. Human Molecular Genetics, 28(12), 1931-1946.

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Visualizing Fungal Root Colonization

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Inoculated A. thaliana roots were observed through confocal microscopy. To trace the colonization process of Ct3-GFP or Ct4-GFP in the roots, we utilized a confocal laser scanning microscope (Nikon C2 plus) equipped with filters optimized for visualization of GFP or mCherry to distinguish fungal hyphae and plant plasma membranes. Fungal lectin staining was performed using a confocal laser scanning microscope Olympus FV1000 with filter settings suitable for fluorescein (FITC), i.e., blue excitation light. WGA-FITC conjugate (Sigma) was used to specify the fungal cell walls. Fungal-inoculated roots were incubated overnight in a 1:3 mixture of chloroform and ethanol. Then, the roots were transferred to chloral hydrate (2 g/mL in water) and incubated for 2 h. After PBS washed roots, the roots were incubated in wheat germ agglutinin (WGA) conjugated to a FITC solution (5 µg/ml; Sigma) for 1 h at room temperature.

Hiruma K., Aoki S., Takino J., Higa T., Utami Y.D., Shiina A., Okamoto M., Nakamura M., Kawamura N., Ohmori Y., Sugita R., Tanoi K., Sato T., Oikawa H., Minami A., Iwasaki W, & Saijo Y. (2023). A fungal sesquiterpene biosynthesis gene cluster critical for mutualist-pathogen transition in Colletotrichum tofieldiae. Nature Communications, 14, 5288.

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