P ask1

Manufactured by Abcam

Sourced in United States

P-ASK1 is a phosphorylated form of ASK1 (Apoptosis Signal-regulating Kinase 1), a serine/threonine protein kinase involved in cellular stress response pathways. It acts as a biomarker for the activation of ASK1.

Automatically generated - may contain errors

Lab products found in correlation

Facsverse, by FlowJo (1 mentions) Transcription factor buffer set, by BD (1 mentions) Trypsin, by Beyotime (1 mentions) Radioimmunoprecipitation assay ripa buffer, by Merck Group (1 mentions) Dcfh da, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Hoechst 33342, by Merck Group (1 mentions) Caspase 3 colorimetric assay kit, by Keygen Biotech (1 mentions)

2 protocols using p ask1

Phosphorylated ASK1 Quantification

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For collection, TC-1 cells were washed with PBS, then trypsin (Beyotime, China) was used. The cells were incubated with Fix/Permed by in a Transcription Factor Buffer Set (BD, 562574) at room temperature in the dark for 15 min.
Subsequently, the cells were incubated with p-ASK1 (Abcam, ab278785) for 30 min at room temperature in the dark for 30 min. BD Biosciences FACSVerseTM was used to test all the samples, and FlowJo 10.4 software was used to analyze the results.

Tang R., Mei S., Xu Q., Feng J., Zhou Y., Xing S., He Z, & Gao Y. (2022). ASK1-ER stress pathway-mediated fibrotic-EV release contributes to the interaction of alveolar epithelial cells and lung fibroblasts to promote mechanical ventilation-induced pulmonary fibrosis. Experimental & Molecular Medicine, 54(12), 2162-2174.

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Apoptosis Pathway Modulation by BBR

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The A549 and PC9 cells were provided by the Cell Bank of Shanghai Institute of Cell Biology, Shanghai, China. BBR was obtained from Sigma and was dissolved at a concentration of 40 mM in dimethyl sulfoxide (DMSO; Sigma-Aldrich) as a stock solution (stored at −20 ℃). It was then diluted to working concentrations with cell culture medium. The antibodies specific for coxIV, p-JNK, JNK, p-ASK1, ASK1, Bax, Bcl-2, caspase-3, cytochrome c, and β-actin were provided by Abcam (Boston, MA, USA). The horseradish peroxidase (HRP)-labeled anti-mouse and anti-rabbit IgG were provided by Santa Cruz Biotechnology Inc. (Dallas, TX, USA). The radioimmunoprecipitation assay (RIPA) buffer, Hoechst33342, and DCFH-DA were provided by Sigma-Aldrich Inc. (Saint Louis, MO, USA). The JC-1 was provided by Life Technologies Inc. (Carlsbad, California, USA). A Caspase-3 Colorimetric Assay Kit was provided by Nanjing Keygen Biotech Co., Ltd. (Nanjing, China). A High Pure Mitochondria Isolation Kit was provided by Shanghai Genmed Biotech Co., Ltd. (Shanghai, China). All the other materials were provided by Bio-Rad (Hercules, CA, USA).

Chen Q., Hou Y., Li D., Ding Z., Xu X., Hao B., Xia Q., Li M, & Fan L. (2022). Berberine induces non-small cell lung cancer apoptosis via the activation of the ROS/ASK1/JNK pathway. Annals of Translational Medicine, 10(8), 485.

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