Standards of rutin and ent-phyllanthidine were obtained from Sigma-Aldrich. Reagents and solvents of analytical grade were purchased from Fluka Chemicals (Busch, Switzerland) and Acros Organics (Hamilton, NJ), respectively. Pre-coated reverse phase HPTLC glass plates of silica gel 60 RP-18F254 were purchased (E. Merck, Germany). The standard and the extracts were applied to RP-HPTLC plates band wise with the help of CAMAG automatic TLC sampler-4 and developed in automatic development chamber-II. Scanning and documentation of developed RP-HPLTLC plate were done by CATS 4 and CAMAG TLC Reprostar 3, respectively.
Standards of rutin
Manufactured by Merck Group
Standards of rutin are reference materials used to calibrate and validate analytical methods for the measurement of rutin content in various samples. Rutin is a flavonoid compound that is commonly found in plant-based materials. These standards provide a consistent and reliable means of quantifying rutin levels across different analytical platforms and laboratories.
Automatically generated - may contain errors
Lab products found in correlation
Automatic tlc sampler 4, by CAMAG (1 mentions)
Tlc reprostar 3, by CAMAG (1 mentions)
Dichloromethane, by Thermo Fisher Scientific (1 mentions)
Acetonitrile of hplc grade, by Thermo Fisher Scientific (1 mentions)
Propan 2 ol, by Thermo Fisher Scientific (1 mentions)
Piperine, by Merck Group (1 mentions)
Curcumin, by Cayman Chemical (1 mentions)
Milli q system, by Merck Group (1 mentions)
Naringenin, by Merck Group (1 mentions)
Sodium carboxymethyl cellulose, by Merck Group (1 mentions)
1200 series hplc, by Agilent Technologies (1 mentions)
2 protocols using standards of rutin
1
Rutin and ent-Phyllanthidine Quantification
2
HPLC Analysis of Bioactive Compounds
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Dichloromethane, ethyl acetate, methanol, propan-2-ol and acetonitrile of HPLC-grade were purchased from Fisher Chemicals. Water for HPLC analysis was prepared by a Milli-Q system (Millipore) and passed through a 0.22 µm membrane filter. Standards of rutin (≥95%) and naringenin (≥95%) were purchased from Sigma-Aldrich. Curcumin (≥90%) was obtained from Cayman Chemicals, and piperine (≥97%), sodium carboxymethyl cellulose as well as AFB1 (≥98%) from Sigma-Aldrich. Formic acid and trifluoroacetic acid were supplied by Acros Organics.
All analyses were performed with an HPLC 1200 series with degasser, quaternary pump, automatic liquid sampler, thermostatic column compartment and diode array detector (DAD) from Agilent. Different HPLC columns were tested: XBridge C18 (5 µm, 4.6 x 250 mm) and Kinetex EVO C18, (5 µm, 4.6 x 250 mm). Water with 0.1% formic acid, water with 0.01% trifluoracetic acid, methanol and acetonitrile were evaluated as mobile phases.
All analyses were performed with an HPLC 1200 series with degasser, quaternary pump, automatic liquid sampler, thermostatic column compartment and diode array detector (DAD) from Agilent. Different HPLC columns were tested: XBridge C18 (5 µm, 4.6 x 250 mm) and Kinetex EVO C18, (5 µm, 4.6 x 250 mm). Water with 0.1% formic acid, water with 0.01% trifluoracetic acid, methanol and acetonitrile were evaluated as mobile phases.
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