Cytation 3 cell imaging multi mode

MMP was evaluated using two fluorescent probes: MitoTracker Red CMXRos (Molecular Probes, Eugen, OR, USA), capable of labeling all mitochondria that conserve MMP, and MitoTracker Green CMXRos (Molecular Probes, Eugen, OR, USA), which labels mitochondria independently of the MMP.
Cells were seeded and treated in 96-well plates (Sarstedt, Nümbrecht, Germany). After the treatment time, cells were incubated for 30 min in media with 200 nM MitoTracker Red CMXRos and 200 nM de MitoTracker Green CMXRos. Finally, fluorescence was quantified using Cytation 3 Cell Imaging Multi-mode (BioTek, Winooski, VT, USA).

Total phenolic content (TPC) [16 ], total flavonoid content (TFC) [17 (link)], and total carotenoid content (TCC) [18 (link)] were determined in the free-conventional, free-eutectic and bound phytochemical fractions from PPBP and PPDF after eutectic extraction. More information can be found in Supplementary Materials and Methods. Results were expressed as mg of gallic acid equivalents per g (mg GAE/g), mg of quercetin equivalents per g (mg QE/g), and mg of β-carotene equivalents per g (mg βCE/g), respectively. The absorbance of samples was determined by a microplate reader (Cytation™ 3 Cell Imaging Multi-Mode, BioTek, Winooski, VT, USA). In addition, the total tannin content (TTC) was also determined in samples using a method described in [19 ], and the results were expressed in mg of cyanidin-3 glucoside equivalents per g (mg C3GE/g) of sample. Phytochemicals in the eutectic extract content were expressed on a per-sample basis, which was calculated based on the weight of the sample used for the extraction.