Peroxidase conjugated sheep anti mouse igg

Manufactured by GE Healthcare

Peroxidase-conjugated Sheep Anti Mouse IgG is a secondary antibody used in various immunoassays and detection techniques. It is produced by immunizing sheep with mouse immunoglobulin G (IgG) and conjugating the resulting antibodies with the enzyme peroxidase. This product can be used to detect and quantify mouse IgG in samples.

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Lab products found in correlation

Sigenome non targeting pool 2, by Horizon Discovery (1 mentions) Ve 821, by Selleck Chemicals (1 mentions) Azd6738, by Selleck Chemicals (1 mentions) Supersignal west pico enhanced chemiluminescence reagent, by Thermo Fisher Scientific (1 mentions) Affinipure bovine anti goat igg, by Jackson ImmunoResearch (1 mentions) Sigenome smartpool, by Horizon Discovery (1 mentions) Anti gapdh, by Merck Group (1 mentions) Peroxidase conjugated goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Histone 3, by Abcam (1 mentions) Proliferating cell nuclear antigen (pcna), by Santa Cruz Biotechnology (1 mentions) C myc, by Santa Cruz Biotechnology (1 mentions)

2 protocols using peroxidase conjugated sheep anti mouse igg

Antibody Validation and siRNA Knockdown for DNA Repair Proteins

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The following antibodies were used in this study at the indicated dilutions: anti-CIP2A (CST #14805; 1 : 1000), anti-GAPDH (Sigma-Aldrich G9545; 1 : 20 000), anti-GFP (gift from Laurence Pelletier, 1 : 10 000), anti-KAP1 (Bethyl A300-274A, 1 : 5000), anti-POLE3 (Bethyl A301-245A-1; 1 : 2000), anti-POLE4 (Abcam ab220695; 1 : 200), anti-Tubulin (Millipore CP06, 1 : 2000), anti-pCHK1 (S345) (Cell Signaling #2348, 1 : 1000), anti-CHK1 (Santa Cruz sc8408, 1 : 500), anti-pRPA32 (S33) (Bethyl A300-246A-3, 1 : 20 000), anti-RPA32 (Abcam ab2175, 1 : 500). The following secondary antibodies for immunoblotting were used in this study: peroxidase-conjugated AffiniPure Bovine Anti-Goat IgG (Jackson Immuno Research 805-035-180) and peroxidase-conjugated Sheep Anti Mouse IgG (GE Healthcare NA931 V). All peroxidase-conjugated secondary antibodies were used at a dilution of 1 : 5000. Protein bands were detected using the SuperSignal West Pico enhanced chemiluminescence reagent (Thermo Fisher Scientific). The following siRNAs from Dharmacon were used in this study: control, siGENOME Non-targeting Pool #2 (D-001206-14-05); POLE3, siGENOME SMARTpool (M-008460-01-0005); POLE4, siGENOME SMARTpool (M-009850-01-0005); APEX2, siGENOME SMARTpool (M-013730-00-0005). ATR inhibitors VE-821 and AZD6738 were purchased from SelleckChem.

Hustedt N., Álvarez-Quilón A., McEwan A., Yuan J.Y., Cho T., Koob L., Hart T, & Durocher D. (2019). A consensus set of genetic vulnerabilities to ATR inhibition. Open Biology, 9(9), 190156.

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Recombinant REV1 Protein Expression and Purification

Cited 1 time

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His-REV1-NT300, corresponding to Xenopus laevis REV1 (MGC: 83743) nucleotide 246-1145, was cloned into the pET28a expression vector. Recombinant His-REV1-NT300 was expressed and purified from DE3 bacteria cells. Anti-REV1 antibodies were raised in rabbit against His-REV1-NT300 (Cocalico Biologicals). Wild type full length (WT) Myc-tagged REV1 corresponding to REV1 nucleotide 246-3938 was cloned into the pCS2+MT vector. ΔUBM1, ΔUBM2, and ΔCTD Myc-tagged REV1 variants were derived from WT Myc-REV1, in which nucleotides 2991-3086, 3225-3327, and 3513-3834 were removed, respectively. Recombinant WT and deletion versions of Myc-tagged REV1 were expressed in SP6 TnT transcription/translation coupled quick master mix kit (Promega). Antibodies against Xenopus ATR, ATRIP, TopBP1, Rad9, RPA32, and Orc2 have been described previously [10 (link),14 (link)]. Additionally, antibodies against Chk1 P-S344 (Cell Signaling), Chk1 (Santa Cruz), c-Myc (Santa Cruz), GST (Santa Cruz), Histone 3 (Abcam), and PCNA (Santa Cruz) were purchased from respective vendors. Peroxidase-conjugated monoclonal mouse anti-rabbit IgG light chain specific (Jackson ImmunoResearch), peroxidase-conjugated goat anti-rabbit IgG (Thermo), and peroxidase-conjugated sheep anti-mouse IgG (GE Healthcare) were used as secondary antibodies in immunoblotting analysis as appropriate.

DeStephanis D., McLeod M, & Yan S. (2015). REV1 is important for the ATR-Chk1 DNA damage response pathway in Xenopus egg extracts. Biochemical and biophysical research communications, 460(3), 609-615.

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