Itgb3

Manufactured by BioLegend

ITGB3 is a protein that plays a role in cell adhesion and signaling. It is a subunit of the integrin receptor complex, which is involved in the binding of cells to extracellular matrix proteins. ITGB3 is expressed on a variety of cell types, including platelets, endothelial cells, and some immune cells.

Automatically generated - may contain errors

Lab products found in correlation

Itgb1, by Thermo Fisher Scientific (1 mentions) Facscalibur flow cytometry system, by BD (1 mentions) Hla dr, by BD (1 mentions) Ssea 1, by Thermo Fisher Scientific (1 mentions) Facscanto 2 cytometer, by BD (1 mentions) Facsdiva software, by BD (1 mentions) Facsaria 3 cell sorter, by BD (1 mentions)

3 protocols using itgb3

Integrin Expression Profiling by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flow cytometry was performed as described [12 (link)]. Following antibodies were used: ITGAV (327,907, BioLegend), ITGB1 (11–0299, Thermo Fisher Scientific), ITGB3 (336,403, BioLegend), ITGB5 (11–0497-42, Thermo Fisher Scientific), ITGB6 (FAB4155P, R&D), HLA-DR (347,401, BD). Stained cells were subjected to FACS Calibur Flow Cytometry System (BD).

Kemper M., Schiecke A., Maar H., Nikulin S., Poloznikov A., Galatenko V., Tachezy M., Gebauer F., Lange T., Riecken K., Tonevitsky A., Aigner A., Izbicki J., Schumacher U, & Wicklein D. (2021). Integrin alpha-V is an important driver in pancreatic adenocarcinoma progression. Journal of Experimental & Clinical Cancer Research : CR, 40, 214.

+ Open protocol

+ Expand

Purification and Characterization of PGCLCs

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

PGCLCs were purified by cell sorting using antibodies against ITGB3 (Biolegend) and SSEA1 (eBioscience) conjugated with PE and Alexa Fluor 647, respectively, as previously described (35 (link),36 ). After dissecting single cells, the cells were resuspended in staining buffer (PBS + 3% FBS) for 20 min on ice, stained with the primary antibodies, washed with staining buffer, resuspended in staining buffer and sorted by FACS. Small debris and doublets were removed by gating for size. For negative controls, we analyzed unstained and secondary antibody only (primary omitted) stained cells. The data were processed using FlowJo software (BD Biosciences). Results were from at least three independent replicates. Statistical significance was determined using the paired Student's t‐test.

Tan K, & Wilkinson M.F. (2022). Regulation of both transcription and RNA turnover contribute to germline specification. Nucleic Acids Research, 50(13), 7310-7325.

+ Open protocol

+ Expand

Sorting and Quantification of PGCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sorting and quantification of PGCLC was performed according to a previously described protocol (Hayashi and Saitou, 2013 (link)). After four days of induction, PGCLC were stained with antibodies against ITGB3 (104307, Biolegend) and SSEA1 (50-8813-41, eBioscience) conjugated with PE and Alexa Fluor 647, respectively. PGCLC quantification was performed with a FACSCantoII Cytometer (BD Bioscience) equipped with BD FACSDiva Software. PGCLC sorting was performed on a FACSAriaIII cell sorter (BD Bioscience).
RNA-seq and ChIP-seq datasets generated in this study have been deposited into GEO repository under accession number GSE70547. All primers used in this study are presented in Table S4.

Respuela P., Nikolic M., Tan M., Frommolt P., Zhao Y., Wysocka J, & Rada-Iglesias A. (2016). Foxd3 promotes exit from naïve pluripotency through enhancer decommissioning and inhibits germline specification. Cell stem cell, 18(1), 118-133.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!