Tg 5silms column

Gas-chromatographic separations were executed on a Trace 1310 (Thermo Scientific) equipped with TriPlus RSH auto sampler (Thermo Scientific) and coupled with a TSQ 8000 electron impact (EI) triple quadrupole mass spectrometer (Thermo Scientific). We used a TG-5SILMS column (Thermo Scientific) with the following dimensions: length 30 m; 0.25-mm inner diameter and 0.25-μm film. The column was operated with helium carrier gas using a PTV injector operating with a column flow 1.2 ml min⁻¹ and a split less injection for 1 min. After initial 60 °C the temperature was raised to 320 °C at a rate of 14.5 °C min⁻¹ and then held for 2 min. For cleaning, the temperature was increased to 350 °C and held for 5 min with a flow of 50 ml min⁻¹. After split less time, the split flow was set to 20 ml min⁻¹. The syringe was cleaned twice with 5 μl n-hexane each before injection and rinsed with 1 μl sample before the injection was done. After injection, the syringe was washed five times with ethyl acetate and five times with n-hexane (5 μl each). The GC oven program started at 100 °C for 1 min and the temperature was increased to 320 °C at 5 °C min⁻¹, and held for 3 min. Measurement using the mass spectrometer was begun after 10 min of monitoring. The mass range between 50 and 650 m/z were monitored. The MS transfer line was set to 300 °C as well as the ion source temperature.