Fura-2-acetoxymethyl ester (Fura-2-AM) and 3’,3’,3’-dipropylthiadicarbocyanine iodide [DiSC3(5)] were purchased from Invitrogen (Carlsbad, CA, USA). ω-conotoxin MVIIC, rottlerin, 2-[1-(3-dimethylaminopropyl)indol-3-yl]-3-(indol-3-yl) maleimide (GF109203X), 5,6,7,13-tetrahydro-13-methyl-5-oxo-12H-indolo[2,3-a]pyrrolo[3,4-c]carbazole-12-propanenitrile (Go6976) and N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H89) were purchased from Tocris Bioscience (Bristol, UK). Echinacoside, dantrolene, dl -threo-beta-benzyl-oxyaspartate (dl -TBOA), 7-chloro-5-(2-chloropheny)-1,5-dihydro-4,1-benzothiazepin-2(3H)-one (CGP37157), 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one) (PD98059), ethylene glycol bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA) and all other reagents were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA).
3 3 3 dipropylthiadicarbocyanine iodide disc3 5
Manufactured by Thermo Fisher Scientific
Sourced in United States
3,3,3-Dipropylthiadicarbocyanine iodide [DiSC3(5)] is a fluorescent dye used in laboratory applications. It is a cationic carbocyanine dye with absorption and emission maxima at approximately 652 nm and 674 nm, respectively. The dye can be utilized for various techniques, including flow cytometry, fluorescence microscopy, and membrane potential measurements.
Automatically generated - may contain errors
Lab products found in correlation
Fura 2 acetoxymethyl ester fura 2 am, by Thermo Fisher Scientific (3 mentions)
N 2 p bromocinnamylamino ethyl 5 isoquinolinesulfonamide h89, by Bio-Techne (3 mentions)
Dantrolene, by Bio-Techne (3 mentions)
Bafilomycin a1, by Bio-Techne (3 mentions)
Fura 2 am, by Thermo Fisher Scientific (2 mentions)
Dl tboa, by Bio-Techne (2 mentions)
Cgp37157, by Bio-Techne (2 mentions)
Gf109203x, by Bio-Techne (2 mentions)
4 aminopyridine 4 ap, by Merck Group (2 mentions)
ω conotoxin gvia ω cgtx gvia, by Alomone (2 mentions)
ω agatoxin iva ω aga iva, by Alomone (2 mentions)
Dantrolene, by Merck Group (1 mentions)
ω conotoxin mviic, by Bio-Techne (1 mentions)
Rottlerin, by Bio-Techne (1 mentions)
5 6 7 13 tetrahydro 13 methyl 5 oxo 12h indolo 2 3 a pyrrolo 3 4 c carbazole 12 propanenitrile go6976, by Bio-Techne (1 mentions)
Ethylene glycol bis β aminoethyl ether n n n n tetraacetic acid egta, by Merck Group (1 mentions)
2 2 amino 3 methoxyphenyl 4h 1 benzopyran 4 one pd98059, by Merck Group (1 mentions)
Echinacoside, by Merck Group (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Igepal ca 630, by Merck Group (1 mentions)
5 protocols using 3 3 3 dipropylthiadicarbocyanine iodide disc3 5
1
Fura-2-AM and DiSC3(5) Calcium Signaling
2
Mitochondrial Isolation and Characterization
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PD was purchased from ChemFaces (Wuhan, Hubei, China). Dantrolene, bafilomycin A1, dl -TBOA, CGP37157, PD98059, GF109203X, H89, ω-conotoxin GVIA and ω-agatoxin IVA were obtained from Tocris Cookson (Bristol, UK). 3′,3′,3′-Dipropylthiadicarbocyanine iodide (DiSC3(5)) and Fura-2-AM were purchased from Invitrogen (Carlsbad, CA, USA). EGTA). Nicotinamide adenine dinucleotide phosphate (NADP+), glutamate dehydrogenase (GDH), sodium dodecyl sulfate (SDS) and all other chemical reagents were supplied by Sigma-Aldrich Co. (St. Louis, MO, USA).
Sucrose buffer: 320 mM sucrose, 10 mM HEPES, pH 7.4; HEPES buffer medium (HBM): 10 mM HEPES, 140 mM NaCl, 5 mM KCl, 1 mM MgCl2, 5 mM NaHCO3, 1.2 mM NaH2PO4 and 10 mM glucose and, pH 7.4. Radioimmunoprecipitation assay buffer (RIPA): 50 mM NaCl, 1.0% IGEPAL® CA-630, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0 (Sigma-Aldrich Co., St. Louis, MO, USA). Tris-buffered saline (TBS-T): 20 mM Tris, 150 mM NaCl, 0.1% (w/v) Tween® 20 detergent.
Sucrose buffer: 320 mM sucrose, 10 mM HEPES, pH 7.4; HEPES buffer medium (HBM): 10 mM HEPES, 140 mM NaCl, 5 mM KCl, 1 mM MgCl2, 5 mM NaHCO3, 1.2 mM NaH2PO4 and 10 mM glucose and, pH 7.4. Radioimmunoprecipitation assay buffer (RIPA): 50 mM NaCl, 1.0% IGEPAL® CA-630, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0 (Sigma-Aldrich Co., St. Louis, MO, USA). Tris-buffered saline (TBS-T): 20 mM Tris, 150 mM NaCl, 0.1% (w/v) Tween® 20 detergent.
3
Mitochondrial Calcium Regulation Assay
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DL-TBOA, bafilomycin A1, dantrolene, CGP37157, KN62, H89, and GF109203X were purchased from Tocris (Bristol, UK). 3,3,3-Dipropylthiadicarbocyanine iodide [DiSC3(5)] and fura-2-acetoxymethyl ester (Fura-2-AM) were purchased from Thermo (Waltham, MA, USA).ω-CgTX GVIA and ω-Aga IVA were purchased from Alomone lab (Jerusalem, Israel). 4-AP, dimethylsulfoxide (DMSO), and all other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).
4
Pharmacological Tools for Neuronal Signaling
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Rosmarinic acid (purity > 98%, ChemFace, Wuhan, China), bafilomycin A1 (Tocris Bioscience, Bristol, United Kingdom), dantrolene (Tocris Bioscience), 7-chloro-5-(2-chlorophenyl)-1,5-dihydro-4,1-benzothiazepin-2(3H)-one (CGP37157, Tocris Bioscience), N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H89, Tocris Bioscience), bisindolylmaleimide I (GF109203X, Tocris Bioscience), 1-[N,O-Bis(5-isoquinolinesulfonyl)-N-methyl-l -tyrosyl]-4-phenylpiperazine (KN62, Tocris Bioscience), 3,3,3-dipropylthiadicarbocyanine iodide [DiSC3(5)] (Thermo, Waltham, MA, USA), and fura-2-acetoxymethyl ester (Fura-2-AM, Thermo) were made up in 10 mM stock solution in DMSO. DL-threo-β-benzyloxyaspartate (DL-TBOA, Tocris Bioscienc), ω-conotoxin GVIA (ω-CgTX GVIA, Alomone Lab, Jerusalem, Israel), and ω-agatoxin IVA (ω-Aga IVA, Alomone Lab) were made up in 5 mM stock solution in water. 4-aminopyridine (4-AP, Sigma-Aldrich, St. Louis, MO, USA) were made up in 300 mM stock solution in water.
5
Neurochemical Modulators in Synaptic Signaling
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Eupafolin (purity>99%) was kindly provided by Professor Horng-Huey Ko. 7-chloro-5-(2-chlorophenyl)-1,5-dihydro-4,1-benzothiazepin-2(3H)-one (CGP37157), DL-threo-β-benzyloxyaspartate (DL-TBOA), dantrolene, N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H89), and 1-[N,O-Bis(5-isoquinolinesulfonyl)-N-methyl-L-tyrosyl]-4-phenylpiperazine (KN62) were purchased from Tocris (Bristol, UK). FM-43, 3,3,3-Dipropylthiadicarbocyanine iodide [DiSC3(5)], and fura-2-acetoxymethyl ester (Fura-2-AM) were purchased from Thermo (Waltham, MA, USA). ω-conotoxin GVIA (ω-CgTX GVIA) and ω-agatoxin IVA (ω-Aga IVA) were purchased from Alomone lab (Jerusalem, Israel). CaMKII, p-CaMKII, synapsin I and anti-rabbit IgG horseradish peroxidase-conjugated antibodies were obtained from Cell Signaling Technologies (Beverly, MA, USA). p-synapsin I (Serine 603) antibody was purchased from GeneTex (CA, USA). 4-aminopyridine (4-AP) and all other reagents were purchased from Sigma (St. Louis, MO, USA).
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