Sw 32 swinging bucket rotor

Manufactured by Beckman Coulter

The SW-32 swinging bucket rotor is a laboratory centrifuge accessory designed for high-speed separation of samples. It features a swinging-bucket design that allows the sample containers to align with the axis of rotation during centrifugation, providing efficient separation of components within the samples. The rotor's core function is to facilitate the separation of materials based on differences in their density and sedimentation rates when subjected to centrifugal force.

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Lab products found in correlation

Slhv033rs, by Merck Group (1 mentions) Pspax2, by Addgene (1 mentions) Fugene 6, by Promega (1 mentions)

2 protocols using sw 32 swinging bucket rotor

Lentiviral Vector Production in HEK293T

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Human embryonic kidney–293 T cells were seeded at 90% confluency in 10-cm dishes with fresh media 24 hours before transfection. Cells were transfected with lentiviral expression vector, psPAX2 (Addgene, #12260), and pMD2.6 (Addgene, #12259) using FuGENE 6 (Promega E2691) at a ratio of 1 μg of DNA to 3 μl of FuGENE 6. Media were collected every 24 hours up to 72 hours after transfection. Cell debris was removed by centrifugation at 2000g for 10 min at 4°C and passaged through a 0.45-μm filter (Millipore, SLHV033RS). Viral particles were concentrated by ultracentrifugation at 25,000 rpm in an SW-32 swinging bucket rotor (Beckman Coulter). Viral particles were resuspended in 150 μl of DMEM high glucose and snap-frozen with liquid nitrogen and stored at −80°C.

Hsieh A., Pitarresi J.R., Lerner J., Donahue G., Hsiehchen D., Rustgi A.K, & Zaret K. (2020). Growth of pancreatic cancers with hemizygous chromosomal 17p loss of MYBBP1A can be preferentially targeted by PARP inhibitors. Science Advances, 6(49), eabc4517.

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Viral Particle Purification from Feces

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VLPs from fecal samples were purified as described previously (Reyes, et al, 2010 (link)) with minor modification. Briefly, 5g of fecal samples were resuspended in 25mL PBS and centrifuged three times at 2500 g for 10 min. Final supernatant was sequentially passed through 40μm, 10μm, 1μm and 0.45μm filters to remove residual cells and byproduct and refilled to 25mL with sterile PBS. 4.14g CsCl was added to the filtrate to make the 1.12 g/ml⁻¹ density solution. 5mL of the filtrate solution was deposited on the top of a 15-ml step gradient prepared using 5 ml CsCl solutions with respective densities of 1.7 g ml⁻¹, 1.5 g ml⁻¹ and 1.35 g ml⁻¹ SM buffer. Samples were centrifuged for 3 hours at 60 000 g at 4C using SW32 swinging bucket rotor (Beckman). 1.5g ml⁻¹ layer was recovered from the tube and dialyzed against PBS overnight.

Gogokhia L., Buhrke K., Bell R., Hoffman B., Brown D.G., Hanke-Gogokhia C., Ajami N.J., Wong M.C., Ghazaryan A., Valentine J.F., Porter N., Martens E., O’Connell R., Jacob V., Scherl E., Crawford C., Stephens W.Z., Casjens S.R., Longman R.S, & Round J.L. (2019). Expansion of bacteriophages is linked to aggravated intestinal inflammation and colitis. Cell host & microbe, 25(2), 285-299.e8.

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