Tyrosinase sc 7833

Manufactured by Santa Cruz Biotechnology

Sourced in United States, United Kingdom

Tyrosinase (sc-7833) is a recombinant protein product offered by Santa Cruz Biotechnology. Tyrosinase is an enzyme involved in the production of melanin.

Automatically generated - may contain errors

Lab products found in correlation

P creb, by Cell Signaling Technology (1 mentions) Tyrosinase t3824, by Merck Group (1 mentions) P erk1 2, by Cell Signaling Technology (1 mentions) Caspase 3, by Cell Signaling Technology (1 mentions) β tubulin sc 9104, by Santa Cruz Biotechnology (1 mentions) Plumbagin, by Merck Group (1 mentions) Erk1 2, by Cell Signaling Technology (1 mentions) Ab55667, by Abcam (1 mentions) Ab12039, by Abcam (1 mentions) Complete protease inhibitor, by Merck Group (1 mentions) Polyvinylidene fluoride membrane, by Merck Group (1 mentions)

Close spelling variants of this product

Tyrosinase (24 citations)

2 protocols using tyrosinase sc 7833

Melanogenesis Regulation Pathway Assay

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Plumbagin, α-MSH (M4135), arbutin (A4256), kojic acid (K3125), l-DOPA (333786), and tyrosinase (T3824) purified from mushrooms were purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against MITF (#12590), p-AKT^S473 (#4060), p-AKT^T308 (#13038), p-CREB (#9198), p-ERK1/2 (#4370), ERK1/2 (#9102), γH2AX (#9718), PARP (#5625), and caspase-3 (#9665) were purchased from Cell Signaling Technology (Danvers, MA, USA). Tyrosinase (sc-7833), and β-tubulin (sc-9104) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Oh T.I., Yun J.M., Park E.J., Kim Y.S., Lee Y.M, & Lim J.H. (2017). Plumbagin Suppresses α-MSH-Induced Melanogenesis in B16F10 Mouse Melanoma Cells by Inhibiting Tyrosinase Activity. International Journal of Molecular Sciences, 18(2), 320.

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Melanocyte Protein Expression Analysis

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Cells were lysed in RIPA buffer (1% NP-40; 150 mM NaCl; 10 mM Tris-HCl, pH 8.0; and 1 mM EDTA) with a complete protease inhibitor (Sigma). The proteins were separated by SDS-polyacrylamide gel and then transferred to a polyvinylidene fluoride membrane (Millipore, Burlington, MA). The antibodies against MITF (ab12039) and RAB27 (ab55667) were obtained from Abcam (Cambridge, United Kingdom); Tyrosinase (sc7833) was from Santacruz Biotechnology (Dallas, TX); Myosin 5A (NBP2-93397) was from Novus Biologicals (Centennial, CO); Mlph was from proteintech (Chicago, IL).

Park Y.J., Kim J.C., Kim Y., Kim Y.H., Park S.S., Muther C., Tessier A., Lee G., Gendronneau G., Forestier S., Ben-Khalifa Y., Park T.J, & Kang H.Y. (2023). Senescent melanocytes driven by glycolytic changes are characterized by melanosome transport dysfunction. Theranostics, 13(12), 3914-3924.

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