Glyceraldehyde 3 phosphate dehydrogenase gapdh 14c10

Recombinant human tumor necrosis-α (TNF-α) was from Peprotech (Rocky Hill, NJ). U0126, SB-203580, and bisindolylmaleimide I were from Cayman Chemical (Ann Arbor, MI). BAY 11–7082 was from Focus Biomolecules (Plymouth Meeting, PA). Goat anti-rabbit IgG-HRP was from GE Healthcare Japan Corporation (Tokyo, Japan). Antibodies for phospho-NF-κB p65 (Ser536) (93H1) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (14C10) were from Cell Signaling Technology (Danvers, MA). Fatty acid-free bovine serum albumin (BSA) was from EMD chemicals (San Diego, CA). Dexamethasone (DEX), Dulbecco’s Modified Eagle Medium (DMEM), and other chemicals were purchased from Sigma-Aldrich (St. Louis, MO).

Antibodies against N-cadherin (D4R1H), vimentin (D21H3), β-catenin (D10A8), Smad2/3 (D7G7), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (14C10) were purchased from Cell Signaling Technology (Danvers, MA, USA). Phospho-specific antibodies against Smad2/3 (Ser465/Ser467) were purchased from Cell Signaling Technology. The RPMI 1640 medium was obtained from GE Healthcare Life Sciences (Piscataway, NJ, USA), and FBS was purchased from ATCC (Manassas, VA, USA). Recombinant human TGF-β was purchased from Peprotech (Rocky Hill, NJ, USA).