Primary antibody for ki 67

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Primary antibody for Ki-67 is a laboratory reagent used for the detection of the Ki-67 protein, which is a cellular marker for proliferation. It can be used in various immunohistochemistry and immunocytochemistry applications.

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Lab products found in correlation

Vectashield antifade mounting medium, by Vector Laboratories (1 mentions) C1 laser scanning confocal microscope system, by Nikon (1 mentions) Recombinant human leptin, by R&D Systems (1 mentions) Phospho p70 s6 kinase thr389, by Cell Signaling Technology (1 mentions) Sulforaphane sfn, by Merck Group (1 mentions) Goat anti rabbit secondary antibody a 11037, by Thermo Fisher Scientific (1 mentions) Prolong gold anti fade mountant with dapi p36931, by Thermo Fisher Scientific (1 mentions) Vitros dt slides, by Ortho Clinical Diagnostics (1 mentions)

2 protocols using primary antibody for ki 67

Immunohistochemical Analysis of Cell Proliferation and Apoptosis

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Paraffin-embedded sections (5-μm thick) were deparaffinized, rehydrated, then steamed for 30 min in citrate buffer for antigen retrieval. Sections were blocked with 5% goat serum in Tris-buffered saline with 0.05% Tween 20, incubated with a primary antibody for Ki-67 (1:100, ThermoFisher Scientific, Waltham, MA, USA) overnight, followed by Alexa Fluor 647-conjugated secondary antibody. For detection of apoptosis, ileal sections were stained with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) using the In-Situ Cell Death Detection Kit, TMR red (Roche Diagnostics, Indianapolis, IN, USA), as per the manufacturer’s instructions. All sections were mounted with VECTASHIELD Antifade Mounting Medium (Vector Laboratories, Burlingame, CA, USA) containing DAPI (4′,6-diamidino-2-phenylindole) as a nuclear stain. Slides were visualized with the C1 laser scanning Confocal Microscope System (Nikon, Melville, NY, USA).

Chaaban H., Patel M.M., Burge K., Eckert J.V., Lupu C., Keshari R.S., Silasi R., Regmi G., Trammell M., Dyer D., McElroy S.J, & Lupu F. (2022). Early Antibiotic Exposure Alters Intestinal Development and Increases Susceptibility to Necrotizing Enterocolitis: A Mechanistic Study. Microorganisms, 10(3), 519.

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Sulforaphane Signaling in Cell Lines

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Sulforaphane (SFN) was purchased from EMD Millipore (cat# 574215; Billerica, MA). Recombinant human leptin was purchased from R&D systems Inc. (cat# 398-LP; Minneapolis, MN). VITROS DT-slides were purchased from Ortho Clinical Diagnostics (Rochester, NY). All surgical tools were purchased from Roboz Surgical Instrument (Gaithersburg, MD). The primary antibody for smooth muscle α-actin was purchased from Abcam (cat# ab5694; Cambridge, MA). The primary antibody for Ki-67 was purchased from Thermo Scientific (cat# RM-9106-S1; Hanover, IL). The primary antibodies for cyclin D1, phospho-Akt (Ser473), phospho-ribosomal protein S6 (Ser235/236) and phospho-p70S6kinase (Thr389) were purchased from Cell Signaling Technology (Danvers, MA). Goat anti-rabbit secondary antibody (A-11037) and prolong gold anti-fade mountant with DAPI (P36931) were purchased from Life Technologies (Grand Island, NY). All other chemicals were from Fisher Scientific (Fair Lawn, NJ) or Sigma-Aldrich (St. Louis, MO).

Shawky N.M., Pichavaram P., Shehatou G.S., Suddek G.M., Gameil N.M., Jun J.Y, & Segar L. (2016). Sulforaphane improves dysregulated metabolic profile and inhibits leptin-induced VSMC proliferation: implications toward suppression of neointima formation after arterial injury in western diet-fed obese mice. The Journal of nutritional biochemistry, 32, 73-84.

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