Plasmid Myc Vif was made by cloning pNL4-3–derived gene vif in pCMV-Myc plasmid from Clontech, United States, as described earlier (Arora et al., 2014 (link)). pBlue3′LTR-luc was obtained from NIH AIDS Reference and Reagent Program of NIH, MD, United States. Glutathione S-transferase (GST) Tat was generated by cloning pNL4-3 derived tat gene in pGEX-4T1 vector from Addgene. HA Tat and Flag Tat were purchased from Addgene. HA Mdm2 was purchased from Sino Biologicals, United States. HA AKT, HA KD AKT (K179A), and HA Myr AKT were kind gifts from Hui Kuan Lin, MD Anderson Cancer Center, TX, United States. Renilla luciferase plasmid was a kind gift from Vivek Natrajan, IGIB, Delhi, India. His Ub plasmid was gifted by Dimitris Xirodimas, University of Dundee. Chemicals used were AKTi (Sigma, United States), PMA (Sigma, United States), IPTG (Sigma, United States), cycloheximide (Sigma, United States), and MG132 (Sigma, United States).
Ha mdm2
Manufactured by Sino Biological
Sourced in United States
HA Mdm2 is a recombinant protein product produced by Sino Biological. It is a tagged version of the human Mdm2 (murine double minute 2) protein, which is a key negative regulator of the tumor suppressor p53. The HA tag allows for easy detection and purification of the protein.
Automatically generated - may contain errors
Lab products found in correlation
Ha tat, by Addgene (2 mentions)
Isopropyl β d 1 thiogalactopyranoside (iptg), by Merck Group (2 mentions)
Mg132, by Merck Group (2 mentions)
Cycloheximide (chx), by Merck Group (2 mentions)
Pcmv myc plasmid, by Takara Bio (1 mentions)
Pgex 4t 1, by Addgene (1 mentions)
Phorbol myristate acetate (pma), by Merck Group (1 mentions)
Pcmv ha vector, by Takara Bio (1 mentions)
Pgex 4t 1 vector, by Addgene (1 mentions)
Insulin, by Merck Group (1 mentions)
2 protocols using ha mdm2
1
Plasmid Construction and Reagent Details
2
Cloning and Expression of Viral Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Viral genes were obtained as previously described (14) . HA-APOBEC3G was cloned using cDNA from TZMbl cells in pCMV-HA vector from Clontech, USA. pBlue3'LTR-luc was obtained from NIH AIDS Reference and Reagent Program of NIH, MD, USA. GST Tat was generated by cloning pNL4-3 derived tat gene in pGEX-4T1 vector from Addgene. HA Tat and Flag NQO1 were purchased from Addgene. HA Mdm2 was purchased from Sino Biologicals, USA. Myc -Vif (T20A) was made from wild type by site directed mutagenesis. HA-AKT, HA-KD-AKT (K179A), HA-Myr-AKT, GST AKT were a kind gift from Hui Kuan Lin, MD Anderson cancer centre, Texas. Renilla luciferase plasmid was a kind gift from Vivek Natrajan, IGIB, Delhi, India. His Ub plasmid was gifted by Dimitris Xirodimas, University of Dundee. Chemicals used were AKTi (Sigma), IPTG (Sigma) and Insulin (Sigma), Cycloheximide (Sigma) and MG132 (Sigma).
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