Vero cells (ATCC, CCL-81), PK15 cells (ATCC, CCL-33), Panc-1 (ATCC, CRL-1469) and primary rat embryonic fibroblasts (REFs) were all maintained in Dulbecco’s Modified Eagle’s Media (DMEM, Cytiva) supplemented with 10% FBS (Omega Scientific) and 1% penicillin-streptomycin (Hyclone), and incubated in a 5% CO2 incubator at 37°C.
REFs were collected from E16–17 Sprague-Dawley rat embryos (Charles River Laboratories), as follows: Animal work was performed in accordance with all applicable regulations and guidelines, and with the approval of the Institutional Animal Care and Use Committee (IACUC) at Arizona State University (protocol #20–1799R). The animal care and use program at Arizona State University has an assurance on file with the Office of Laboratory Animal Welfare (OLAW), is registered with the USDA, and is accredited by AAALAC International. Briefly, embryos were decapitated and internal organs removed. Remaining skin and connective tissue was trypsinized (Trypsin-EDTA, Gibco) at 37°C, pipetted vigorously in complete DMEM, and supernatants were plated onto 10cm cell culture dishes (Celltreat). REFs were passaged no more than 4 times before use in experiments [76 ].
REFs were collected from E16–17 Sprague-Dawley rat embryos (Charles River Laboratories), as follows: Animal work was performed in accordance with all applicable regulations and guidelines, and with the approval of the Institutional Animal Care and Use Committee (IACUC) at Arizona State University (protocol #20–1799R). The animal care and use program at Arizona State University has an assurance on file with the Office of Laboratory Animal Welfare (OLAW), is registered with the USDA, and is accredited by AAALAC International. Briefly, embryos were decapitated and internal organs removed. Remaining skin and connective tissue was trypsinized (Trypsin-EDTA, Gibco) at 37°C, pipetted vigorously in complete DMEM, and supernatants were plated onto 10cm cell culture dishes (Celltreat). REFs were passaged no more than 4 times before use in experiments [76 ].