Au5800 automated

The complete blood count and CRP were detected using a 6800-plus (Mindray, China) automatic blood cell analyzer and supporting reagents. The AU5800 automated biochemical analyzer (Beckman Coulter, USA) and supporting reagents detected biochemical indicators. The coagulation function was detected using the STAGO STA-R Max (Stago, France) automatic coagulation analyzer and supporting reagents. PCT was detected using the Roche Infinity (Roche Diagnostics, Germany) electrochemiluminescence Analyzer and its supporting reagents.
Measurement of cytokine levels (IL-2, IL-4, IL-6, IL-10, TNF- α and IFN- γ) and lymphocyte subsets using flow cytometry (FACS Canto TM II, BD, New Jersey, USA). The percentage of lymphocyte subsets was calculated using BD FACSCanto clinical software and BD FACSDiva software.

The following clinical variables were obtained from each patient from electronic medical records (EMRs): age, sex, trauma mechanism, systolic blood pressure (mmHg), respiratory and pulse rate, body temperature (BT, °C), and oxygen saturation on admission, initial Glasgow Coma Scale (GCS) score, transfusion amount of packed red blood cells (PRC) during the first 24-h after admission, in-hospital mortality, and 24-h mortality. Laboratory results, including albumin and lactate levels, were obtained at the time of arrival at the ED. The L-lactate level was measured using an enzymatic method with an AU5800 automated chemistry analyzer (Beckman Coulter, Brea, CA, USA). The AIS and ISS were calculated using information from the EMRs. LAR was calculated as the ratio of lactate level to albumin level. MT was defined as transfusion of >10 units of PRCs within the first 24 h of admission or >4 units within 1 h of admission.[12 (link)] The primary outcome was 24-h mortality, and the secondary outcome was MT.