Progoitrin

Manufactured by Phytolab

Sourced in Germany

Progoitrin is a naturally occurring compound found in certain plants. It is a member of the glucosinolate family of compounds. Progoitrin functions as a precursor to other biologically active compounds, but its core function is not explicitly stated.

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Lab products found in correlation

Gluconapin, by Phytolab (3 mentions) Glucoerucin, by Phytolab (3 mentions) Glucoraphenin, by Phytolab (3 mentions) Glucobrassicin, by Phytolab (3 mentions) Glucoraphanin, by Phytolab (3 mentions) Sinigrin, by Phytolab (2 mentions) Gluconasturtiin, by Phytolab (2 mentions) Milli q purification system, by Merck Group (2 mentions) Glucotropaeolin, by Phytolab (2 mentions) Glucobrassicanapin, by Phytolab (2 mentions) Glucoiberin, by Phytolab (2 mentions) Lc ms grade methanol, by Thermo Fisher Scientific (1 mentions) Lc ms grade formic acid, by Thermo Fisher Scientific (1 mentions) Hplc grade methanol, by Thermo Fisher Scientific (1 mentions) Sinalbin, by Phytolab (1 mentions) Milli q, by Merck Group (1 mentions) Lc ms grade acetonitrile, by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) Formic acid, by Merck Group (1 mentions)

4 protocols using progoitrin

Quantification of Glucosinolates in Broccoli

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Broccoli seeds (BHL106) were obtained from the Vegetable Research Center of the Beijing Academy of Agricultural and Forestry Sciences. Methanol, formic acid, ammonium acetate, acetonitrile, Sinigrin (SIN), Gluconapin (NAP), Progoitrin (PRO), Glucotropaeolin (TRO), Glucoerucin (ERU), Gluconasturtiin (NAS), Glucoraphenin (RAE), Glucobrassicin (GBC), Glucoalyssin (ALY), 4-MethoxyGlucobrassicin (4ME), NeoGlucobrassicin (NEO), and glucoraphanin were purchased from PhytoLab GmbH & Co. KG (Vestenbergsgreuth, Middle Franconia, Germany). The purity of all chemical substances was >99%. Individual stock solutions of the 13 GSLs were prepared in 70% methanol. Ultrapure deionized water (18.2 MΩ) was prepared using Milli-Q purification systems (Millipore, MA, USA).

Liu G., He H., Wang P., Zhao X, & Ren F. (2023). Glucoraphanin Accumulation via Glucoraphanin Synthesis Promotion during Broccoli Germination. Foods, 13(1), 41.

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Quantitative Analysis of Glucosinolates

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High-purity (>98%) GSL standards were purchased from PhytoLab GmbH & Co. KG (Vestenbergsgreuth, Middle Franconia, Germany): sinigrin (SIN), gluconapin (NAP), glucobrassicanapin (GBN), progoitrin (PRO), glucoerucin (ERU), glucoraphenin (RAE), glucoraphanin (RAA), glucobrassicin (GBC), glucotropaeolin (TRO) and gluconasturtiin (NAS). 4-Methoxyglucobrassicin (4ME) and neoglucobrassicin (NEO) were purchased from Extrasynthese (Z.I Lyon Nord, Genay Cedex France) and glucoalyssin (ALY) was purchased from Shanghai ZZBIO Co., Ltd. (Shanghai, China). HPLC-grade methanol, LC/MS-grade methanol and LC/MS-grade formic acid were supplied by Fisher Scientific (Pittsburgh, PA, USA). Ultrapure water was obtained using Milli-Q purification systems (Millipore, MA, USA). Individual stock solutions of the 13 GSLs were prepared in 70% methanol.

Yu X., He H., Zhao X., Liu G., Hu L., Cheng B, & Wang Y. (2021). Determination of 18 Intact Glucosinolates in Brassicaceae Vegetables by UHPLC-MS/MS: Comparing Tissue Disruption Methods for Sample Preparation. Molecules, 27(1), 231.

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Comprehensive GSL Compound Analysis

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All the reagents employed for both extraction and analysis in this study were analytical-grade products obtained from Sigma-Aldrich (St. Louis, MO, USA) and ThermoFisher Scientific Korea (Seoul, Republic of Korea). Among the 17 GSL standards, 6 GSLs including Progoitrin (PRO), EpiProgoitrin (EPI), Glucobrassicanapin (GBN), Glucoiberin (GIB), Glucoraphenin (GRE), and Sinalbin (SNB) were purchased from Phytolab (Martin Baue, KG, Germany) and the remaining 11 GSL were purchased from Phytoplan (Neuenheimer, Heidelberg, Germany). All standards had a purity of ≥98%.

Kim S.H., Subramanian P, & Hahn B.S. (2023). Glucosinolate Diversity Analysis in Choy Sum (Brassica rapa subsp. chinensis var. parachinensis) Germplasms for Functional Food Breeding. Foods, 12(12), 2400.

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Glucosinolate Quantification Protocols

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LC-MS grade acetonitrile, methanol and formic acid, were obtained from Sigma-Aldrich Chemical Company (St Louis, MO). HPLC grade water (18 mV) was produced using a Millipore Milli-Q (Bedford, MA, USA) purification system. Glucosinolate standards (glucobrassicin, glucoraphanin, glucoiberin, gluctropaeolin, glu-conapin, progoitrin, glucoerucin and glucoraphenin potassium salts) were purchased from PhytoLab GmbH & Co. KG (Vesten-bergsgreuth, Germany). Standard purity was more than 80%.

Maldini M., Foddai M., Natella F., Petretto G.L., Rourke J.P., Chessa M, & Pintore G. (2017). Identification and quantification of glucosinolates in different tissues of Raphanus raphanistrum by liquid chromatography tandem-mass spectrometry. Journal of food composition and analysis : an official publication of the United Nations University, International Network of Food Data Systems, 61.

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