Free t3 elisa kit

The rat neuronal-like NeuroScreen 1 (NS-1) and rat hepatic H4IIE cell lines were from American Type Culture Collection (ATCC; Manassas, VA). RPMI 1640 medium, low glucose Dulbecco’s modified Eagle’s medium (LG-DMEM), antibiotic (penicillin/streptomycin) solution, phosphate-buffered saline (PBS), L-glutamine, activated charcoal, Dowex (AG-1X-10) resin, Ponceau S Stain, Cell Viability Green Indicator dye, reverse transcriptase system, fetal bovine serum (FBS), and the SYBR Green PCR Master Mix were purchased from Thermo Scientific (Logan, UT). NGF was from Fisher Scientific (Pittsburg, PA). 3,5,3’-Triiodothyronine (T3) was from Sigma–Aldrich (St. Louis, MO). The free T3 ELISA kit was obtained from Fitzgerald (Acton, MA). TRI Reagent was purchased from Molecular Research Center (Cincinnati, OH). The Turbo DNA-free kit was acquired from Ambion (Austin, TX). Rabbit anti-TRα/β (FL-408; diluted 1:100), goat anti-amyloid A4 (diluted 1:100), and goat anti-actin (diluted 1:250) specific antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Rabbit anti-Aβ (diluted 1:100), sheep anti-tau (diluted 1:333), mouse anti-actin (diluted 1:500), anti-rabbit Dylight 680, anti-mouse Dylight 680, anti-sheep Dylight 680, and anti-goat Dylight 800 were from Pierce Thermo Scientific Pierce (Rockford, IL). All other materials used were from Fisher Scientific or Sigma–Aldrich.