The four different antigens of dsDNA (Sigma, 438545-06-3), cardiolipin (Sigma, C0563), hemocyanin (Sigma, H8283), and insulin (Sigma, I9278) were coated on ELISA plates following the previous conditions34 (link): dsDNA (30 ng/well), cardiolipin (1.5 µg/well), hemocyanin (150 ng/well), and insulin (150 ng/well). The subsequent ELISA procedures were performed as described above.
Hemocyanin
Manufactured by Merck Group
Sourced in United States
Hemocyanin is a naturally occurring, copper-containing respiratory protein found in the hemolymph of certain invertebrates, such as mollusks and arthropods. It functions as an oxygen-carrying molecule, similar to the role of hemoglobin in vertebrates. Hemocyanin is commonly used in laboratory settings as a model system for studying protein structure, function, and interactions.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using hemocyanin
1
ELISA for Autoantibody Detection
2
Fluorescent Biomolecule Detection Protocol
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The main reagents used in this study included: fluorenylmethyloxycarbonyl (Fmoc) amino acids (Amersham Pharmacia Biotech, Piscataway, NJ, USA), hemocyanin (Sigma-Aldrich, Hong Kong, China), calf thymus DNA (DingGuo ChangSheng Biotechnology Co., Ltd., Beijing, China), and dsDNA immunofluorescence assay kit and extractable nuclear antigens (ENA) polypeptide spectrum kit (Euroimmun AG, Lübeck, Germany).
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