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Rs 2 technology

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The RS II technology is a sequencing platform developed by Pacific Biosciences. It utilizes single-molecule, real-time (SMRT) sequencing technology to generate high-quality, long-read sequence data. The core function of the RS II technology is to perform DNA sequencing by detecting the incorporation of fluorescently labeled nucleotides during the synthesis of DNA strands.

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Lab products found in correlation

Hiseq 2500 v4, by Illumina (2 mentions)

Spelling variants of this product

RS II (40 citations) PacBio RS II single-molecule real-time (SMRT) sequencing technology (4 citations) RS II sequencing technology (3 citations) PacBio RS II sequencing technology (2 citations) SMRT Technology PacBio RS II (1 citations)

2 protocols using rs 2 technology

1

Genome Sequencing of Captive Male

Check if the same lab product or an alternative is used in the 5 most similar protocols
We sequenced the genome of an Independent male from the captive colony. Illumina HiSeq 2500 v4 150-bp paired reads were generated, using paired-end and mate-pair libraries with various insert sizes, resulting in 137x raw coverage (Supplementary Table 2, Supplementary Fig. 2). We used Pacific Biosciences RS II technology with P5–C3 chemistry to generate 8.8x coverage in long reads (mean length 5,713 bp).
Küpper C., Stocks M., Risse J.E., dos Remedios N., Farrell L.L., McRae S.B., Morgan T.C., Karlionova N., Pinchuk P., Verkuil Y.I., Kitaysky A.S., Wingfield J.C., Piersma T., Zeng K., Slate J., Blaxter M., Lank D.B, & Burke T. (2015). A supergene determines highly divergent male reproductive morphs in the ruff. Nature genetics, 48(1), 79-83.
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2

Genome Sequencing of Captive Male

Check if the same lab product or an alternative is used in the 5 most similar protocols
We sequenced the genome of an Independent male from the captive colony. Illumina HiSeq 2500 v4 150-bp paired reads were generated, using paired-end and mate-pair libraries with various insert sizes, resulting in 137x raw coverage (Supplementary Table 2, Supplementary Fig. 2). We used Pacific Biosciences RS II technology with P5–C3 chemistry to generate 8.8x coverage in long reads (mean length 5,713 bp).
Küpper C., Stocks M., Risse J.E., dos Remedios N., Farrell L.L., McRae S.B., Morgan T.C., Karlionova N., Pinchuk P., Verkuil Y.I., Kitaysky A.S., Wingfield J.C., Piersma T., Zeng K., Slate J., Blaxter M., Lank D.B, & Burke T. (2015). A supergene determines highly divergent male reproductive morphs in the ruff. Nature genetics, 48(1), 79-83.
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