B cells and naïve CD4+ T cells were isolated from mouse spleen using mouse B cell and naïve T cell isolation kits (Miltenyi Biotech) according to previously published optimized protocols 30 , 31 . NIH3T3 mouse fibroblast cell line (ATCC CRL-1658) was provided by O. Voss. Phoenix Eco retroviral packaging cell line (ATCC CRL-3214) was purchased from American Type Culture Collection (ATCC). All cells were maintained in RPMI 1640 media supplemented with 50 μM 2-mercaptoethanol, 50 U/ml penicillin, 50 μM streptomycin, 2 mM L-glutamine, 0.1 mM non-essential amino acids, 1 mM sodium pyruvate and 10 mM HEPES unless otherwise specified. The pathway inhibitors SP600125, PD98059, Wortmannin, Rapamycin, SB202190 were purchased from Invivogen. MHY1485 and Akt Inhibitor IV (Akt IV) were purchased from Sigma-Aldrich.
Akt inhibitor 4 akt 4
Manufactured by Merck Group
Akt Inhibitor IV (Akt IV) is a laboratory compound that functions as a selective inhibitor of the Akt protein kinase. It is commonly used in research settings to study the role of the Akt signaling pathway in various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Rapamycin, by InvivoGen (2 mentions)
Sb202190, by InvivoGen (2 mentions)
Mouse b cell and na ve t cell isolation kits, by Miltenyi Biotec (2 mentions)
Nih3t3 mouse fibroblast cell line, by American Type Culture Collection (2 mentions)
Phoenix eco retroviral packaging cell line, by American Type Culture Collection (2 mentions)
Pd98059, by InvivoGen (2 mentions)
Sp600125, by InvivoGen (2 mentions)
Mhy1485, by Merck Group (2 mentions)
Wortmannin, by InvivoGen (2 mentions)
2 protocols using akt inhibitor 4 akt 4
1
Isolation and Culture of Murine Immune Cells
2
Isolation and Culture of Murine Immune Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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B cells and naïve CD4+ T cells were isolated from mouse spleen using mouse B cell and naïve T cell isolation kits (Miltenyi Biotech) according to previously published optimized protocols 30 , 31 . NIH3T3 mouse fibroblast cell line (ATCC CRL-1658) was provided by O. Voss. Phoenix Eco retroviral packaging cell line (ATCC CRL-3214) was purchased from American Type Culture Collection (ATCC). All cells were maintained in RPMI 1640 media supplemented with 50 μM 2-mercaptoethanol, 50 U/ml penicillin, 50 μM streptomycin, 2 mM L-glutamine, 0.1 mM non-essential amino acids, 1 mM sodium pyruvate and 10 mM HEPES unless otherwise specified. The pathway inhibitors SP600125, PD98059, Wortmannin, Rapamycin, SB202190 were purchased from Invivogen. MHY1485 and Akt Inhibitor IV (Akt IV) were purchased from Sigma-Aldrich.
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