The protein samples were subjected to 10–20% gradient sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The proteins were stained by Coomassie Brilliant Blue (CBB; Nacalai Tesque, Kyoto, Japan) or transferred to an Immobilon-P Transfer Membrane (Merck Millipore) and reacted with the appropriate antibodies. The immune complexes were visualized with the SuperSignal West Femto substrate (Thermo Scientific, Rockford, IL) and detected by an LAS-4000 image analyzer system (Fujifilm, Tokyo, Japan).
Las 4000 image analyzer system
Manufactured by Fujifilm
Sourced in Japan
The LAS-4000 image analyzer system is a laboratory instrument designed for the analysis and quantification of various types of images. It provides essential tools for researchers and scientists to objectively evaluate and measure parameters within their samples or experimental data.
Automatically generated - may contain errors
Lab products found in correlation
Supersignal west femto substrate, by Thermo Fisher Scientific (2 mentions)
Immobilon p transfer membrane, by Merck Group (1 mentions)
Coomassie brilliant blue cbb, by Nacalai Tesque (1 mentions)
Amersham imager 600, by GE Healthcare (1 mentions)
Polyvinylidene difluoride membrane, by Merck Group (1 mentions)
Complete mini, by Merck Group (1 mentions)
2 protocols using las 4000 image analyzer system
1
SDS-PAGE and Immunoblotting Protocol
2
Western Blot Analysis of Cellular Proteins
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Cells lysed on ice in lysis buffer (20 mM Tris-HCl [pH 7.4], 135 mM NaCl, 1% Triton-X 100, 10% glycerol) supplemented with a protease inhibitor cocktail, cOmplete mini (MilliporeSigma), were boiled in loading buffer and subjected to 5%–20% gradient SDS-PAGE. The proteins were transferred to polyvinylidene difluoride membranes (MilliporeSigma) and incubated with the appropriate antibodies. The immune complexes were visualized with SuperSignal West Femto substrate (Thermo Fisher Scientific). The signals were detected by use of an LAS-4000 image analyzer system (Fujifilm)/an Amersham Imager 600 (GE Healthcare) or an WSE-LuminoGraph I (ATTO)/ImageSaver6 (ATTO).
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