Cells were lysed in buffer containing 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 4 mM EDTA, 1% Nonidet (N) P-40, 0.1% sodium dodecyl sulfate (SDS), 1 mM dithiothreitol (DTT), and 1 mM phenylmethylsulfonyl fluoride (PMSF). Supernatants from these lysates were subjected to SDS-polyacrylamide gel electrophoresis, followed by immunoblot analysis using anti-Rad18 (A301-340A; Bethyl Lab, Inc., Montgomery, TX, USA), anti-ß-actin (A5441; Sigma, Saint Louis, MI, USA), anti-HA (HA-7; Sigma), anti-Myc-tag mAb (PL-14; MBL, Nagoya, Japan) or anti-hORF1p (SE-6798)34 (link) as primary antibodies. We used peroxidase-conjugated Donkey anti-Rabbit IgG (H + L) (Jackson ImmunoResearch) or Amersham ECL peroxidase-linked Sheep anti-Mouse IgG (GE Healthcare) as secondary antibodies. The proteins were detected by using Western Lightning Plus ECL, enhanced chemiluminescence substrate (PerkinElmer, Waltman, MA) and Amersham Imager 600 (GE Healthcare Bio-Sciences, Uppsala, Sweden).
Peroxidase conjugated donkey anti rabbit igg h l
Manufactured by Jackson ImmunoResearch
Peroxidase-conjugated Donkey anti-Rabbit IgG (H + L) is a secondary antibody that binds to primary antibodies raised in rabbits. It is conjugated to the enzyme peroxidase, which can be used to detect and visualize the presence of the primary antibody in various laboratory techniques.
Automatically generated - may contain errors
Lab products found in correlation
Amersham ecl peroxidase linked sheep anti mouse igg, by GE Healthcare (2 mentions)
Anti ha ha 7, by Merck Group (2 mentions)
Anti actin, by Merck Group (1 mentions)
Amersham imager 600, by GE Healthcare (1 mentions)
Western lightning plus ecl, by PerkinElmer (1 mentions)
Goat anti rabbit igg, by LI COR (1 mentions)
Anti flag m2 antibody, by Merck Group (1 mentions)
Anti hrgfp, by Agilent Technologies (1 mentions)
Anti p21 waf1 cip1 12d1, by Cell Signaling Technology (1 mentions)
Irdye 800cw goat anti mouse igg, by LI COR (1 mentions)
Irdye 680rd goat anti mouse igg, by LI COR (1 mentions)
Anti ha, by Santa Cruz Biotechnology (1 mentions)
Irdye 800cw goat anti rabbit igg, by LI COR (1 mentions)
Anti β actin, by Merck Group (1 mentions)
2 protocols using peroxidase conjugated donkey anti rabbit igg h l
1
Immunoblot Analysis of Rad18 Protein
2
Western Blot Analysis of HIV-1 and Cell Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were lysed in buffer containing 50 mM Tris–HCl (pH 8.0), 150 mM NaCl, 4 mM EDTA, 1% Nonidet (N) P-40, 0.1% sodium dodecyl sulfate (SDS), 1 mM dithiothreitol (DTT) and 1 mM phenylmethylsulfonyl fluoride (PMSF). Supernatants from these lysates were subjected to SDS-polyacrylamide gel electrophoresis, followed by immunoblot analysis using anti-HIV-1 p24 (65-005; Bioacademia, Osaka, Japan), anti-p21Waf1/Cip1 (12D1) (#2947; Cell Signaling Technology, Inc., Danvers, MA 01923, USA), anti-p21 [EPR362] (ab 109520; Abcam), anti-β-actin (A5441; Sigma, Saint Louis, MI, USA), anti-HA (HA-7; Sigma), anti-HA (12CA5; Santa Cruz), anti-FLAG antibody (M2; Sigma), anti-S6 ribosomal protein (5G10; Cell Signaling Technology), anti-GAPDH (GA1R, Pierce Biotechnology), anti-hrGFP (#240141, Agilent Technologies), or anti-hORF1p (SE-6798) (65 (link)) as primary antibodies. We used peroxidase-conjugated Donkey anti-Rabbit IgG (H+L) (Jackson ImmunoResearch), Amersham ECL peroxidase-linked Sheep anti-Mouse IgG (GE Healthcare), IRDye 800CW Goat anti-Rabbit IgG, IRDye 800CW Goat anti-Mouse IgG, IRDye 680RD Goat anti-Rabbit IgG or IRDye 680RD Goat anti-Mouse IgG (LI-COR Biosciences) as secondary antibodies.
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