Goat anti rabbit igg apc

The IF staining was carried out as previously reported [24 (link),25 (link),27 ,65 (link),66 ]. Briefly, cells seeded and treated in chamber slides were fixed with 4 % paraformaldehyde for 15 min at RT, treated with 0.5 % Triton X-100 for 20 min, and blocked with 5 % goat serum (1:10 dilution) for 20 min at RT, followed by incubating with primary antibodies against SV40 T (1:50 dilution; Santa Cruz; Cat# sc-147), Krt10 (1:50 dilution; Bimake; Cat# A5266), Krt14 (1:50 dilution; Bimake; Cat# A5434), and Krt15 (1:50 dilution; Bimake; Cat# A5627) overnight. After being washed, the cells were incubated with DyLight 594, goat anti-mouse IgG (1:200 dilution; Abbkine; Cat# A23410) or goat anti-rabbit IgG/APC (1:200 dilution; Bioss; Cat# bs-0295G-APC). The nuclei were counterstained with DAPI (10 μg/mL). Minus primary antibody or control IgG was used as a negative control. IF results were recorded by using a laser confocal microscope (Leica TCS SP8).

The IF staining was carried out as previously described [25 ,37 (link),57 (link)]. Briefly, cells were seeded and treated in chamber slides, fixed with 4 % paraformaldehyde for 15 min at RT, treated with 0.5 % Triton X-100 for 20 min, and blocked with 5 % goat serum (1:10 dilution) for 20 min at RT, followed by incubation with primary antibodies against CD105 (1:100 dilution; Proteintech; Cat# 10862-1-AP), NANOG (1:100 dilution; Proteintech; Cat# 14295-1-AP), ACTA2 (1:50 dilution; Bimake; Cat# A5550), HAND1 (1:100 dilution; Bioworld; Cat# MB63487) or PPARγ (1:100 dilution; Affinity; Cat# AF6284) overnight. After being washed, the cells were incubated with goat anti-rabbit IgG/APC (1:200 dilution; Bioss; Cat# bs-0295G-APC) or CoraLite594 – conjugated Goat Anti-Rabbit IgG (H + L) (1:200 dilution; Proteintech; Cat# SA00013-4) or Fluorescein (FITC)–conjugated Affinipure Goat Anti-Rabbit IgG(H + L) (1:200 dilution; Proteintech; Cat No. SA00003-2). The cell nuclei were counterstained with DAPI (10 μg/mL). Minus primary antibodies were used as negative controls. IF staining results were recorded under a confocal microscope (Leica TCS SP8).