Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrasodium bromide (MTT) absorbance (Sigma Aldrich). For cell cycle analysis c ells (2 × 106 cells/ml) were serum-starved overnight, cultured for 24 h in RPMI-1640/10% FCS medium with additions as indicated, fixated in ethanol, stained with propidium iodide (PI), and subjected to FACScan/CellQuest analysis (BD Biosciences). For apoptosis analysis cells were cultured in RPMI-1640/10% FCS medium with additions as indicated, stained by Annexin V-FITC (BD Pharmingen) and PI, and subjected to FACScan/CellQuest analysis (BD Biosciences).
Facscan cellquest analysis
Manufactured by BD
The FACScan/CellQuest analysis is a flow cytometry system used for cell analysis and sorting. It is designed to measure and analyze the physical and fluorescent characteristics of cells or other particles in a fluid stream.
Automatically generated - may contain errors
Lab products found in correlation
1 protocol using facscan cellquest analysis
1
Cell Viability, Cycle, and Apoptosis Analysis
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