Anti human cd19 brilliant violet 510

For immunophenotyping, nasal cells were dislodged from curettes by pipetting and stained with an antibody cocktail containing anti-human CD3 APC, anti-human CD14 PE-Cy7, anti-human CD66b PE, anti-human CD19 Brilliant Violet 510, and anti-human CD45 Alexa Fluor 700 (all BioLegend, UK). Samples were acquired on an LSR FORTESSA flow cytometer (BD Biosciences, UK) and analyzed using Flowjo v10.5.3 (BD Biosciences, USA).

Nasal microbiopsies were obtained from the inferior turbinate of each participant using microcurettes without local analgesia. Each sample was dropped immediately into transport medium and transferred to the laboratory in less than 10 min. For immunophenotyping, nasal cells were dislodged from curettes by gentle pipetting and suspension. Cells were then live-stained with an antibody cocktail containing anti-human CD3 APC, anti-human CD19 Brilliant Violet 510, TCR Gamma Delta FITC and anti-human CD45 Alexa Fluor 700 (all BioLegend), anti-human CD14 PE-Cy7 (BD Biosciences), anti-human CD66b PE (eBiosciences) and MR1 Tetramer Bv421 (NIH Tetramer Core Facility). After fixation, samples were acquired using standard methods on an LSR FORTESSA flow cytometer (BD Biosciences) and analysed using Flowjo v10.5.3 (BD Biosciences).